LOCUS       SIU01556.1               291 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 probable
            penicillin-binding protein dacb2 (d-alanyl-d-alanine carboxypeptidas
            e) (dd-peptidase) (dd-carboxypeptidase) (pbp) (dd-transpeptidase)
            (serine-type d-ala-d-ala carboxypeptidase) (d-amino acid
            hydrolase) protein.
ACCESSION   LT708304-2970
PROTEIN_ID  SIU01556.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="dacB2"
                     /locus_tag="BQ2027_MB2935"
                     /note="Mb2935, dacB2, len: 291 aa. Equivalent to Rv2911,
                     len: 291 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 291 aa overlap). Probable dacB2,
                     D-alanyl-D-alanine carboxypeptidase (penicillin-binding
                     protein) (EC 3.4.16.4), an ala-rich protein. Highly
                     similar (except in N-terminus) to Q9CCM2|ML0691 PUTATIVE
                     D-ALANYL-D-ALANINE CARBOXYPEPTIDASE from Mycobacterium
                     leprae (411 aa), FASTA scores: opt: 749, E(): 9.3e-39,
                     (46.75% identity in 276 aa overlap). Also similar to
                     penicillin binding proteins / D-alanyl-D-alanine
                     carboxypeptidases e.g. Q9KCJ8|SC4G1.16c D-ALANYL-D-ALANINE
                     CARBOXYPEPTIDASE from Streptomyces coelicolor (382 aa),
                     FASTA scores: opt: 386, E(): 2.1e-16, (31.25% identity in
                     285 aa overlap); P35150|DACB_BACSU PENICILLIN-BINDING
                     PROTEIN 5* PRECURSOR from Bacillus subtilis (382 aa),
                     FASTA scores: opt: 384, E(): 3.6e-17, (30.7% identity in
                     244 aa overlap); Q9K8X5|DACB|BH2877 D-ALANYL-D-ALANINE
                     CARBOXYPEPTIDASE (PENICILLIN-BINDING PROTEIN 5) from
                     Bacillus halodurans (395 aa), FASTA scores: opt: 359, E():
                     9.7e-15, (30.3% identity in 241 aa overlap);
                     P33364|PBP7_ECOLI|PBPG|B2134 penicillin-binding protein 7
                     precursor from Escherichia coli strain K12 (313 aa), FASTA
                     scores: opt: 273, E(): 7.5e-10, (27.8% identity in 263 aa
                     overlap); etc. Also similar to O53380|Rv3330|MTV016.30
                     PENICILLIN-BINDING PROTEIN from Mycobacterium tuberculosis
                     (405 aa), FASTA scores: opt: 746, E(): 1.4e-38, (47.0%
                     identity in 266 aa overlap). Seems to contain PF00768
                     Peptidase_S11 domain PFAM. BELONGS TO PEPTIDASE FAMILY
                     S11; ALSO KNOWN AS THE D-ALANYL-D-ALANINE CARBOXYPEPTIDASE
                     1 FAMILY. Thought to be a membrane-bound protein. Note
                     that previously known as dacB. Protein product from Mb2935
                     detected using SWATH mass spectrometry. Mb2935 found to be
                     expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y3C8"
                     /db_xref="InterPro:IPR001967"
                     /db_xref="InterPro:IPR012338"
                     /db_xref="InterPro:IPR018044"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y3C8"
BEGIN
        1 MRKLMTATAA LCACAVTVSA GAAWADADVQ PAGSVPIPDG PAQTWIVADL DSGQVLAGRD
       61 QNVAHPPAST IKVLLALVAL DELDLNSTVV ADVADTQAEC NCVGVKPGRS YTARQLLDGL
      121 LLVSGNDAAN TLAHMLGGQD VTVAKMNAKA ATLGATSTHA TTPSGLDGPG GSGASTAHDL
      181 VVIFRAAMAN PVFAQITAEP SAMFPSDNGE QLIVNQDELL QRYPGAIGGK TGYTNAARKT
      241 FVGAAARGGR RLVIAMMYGL VKEGGPTYWD QAATLFDWGF ALNPQASVGS L
//