LOCUS SIU01214.1 597 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 LONG CONSERVED HYPOTHETICAL PROTEIN [SECOND PART] protein. ACCESSION LT708304-2628 PROTEIN_ID SIU01214.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB2596" /note="Mb2596, -, len: 597 aa. Equivalent to 3' end of Rv2566, len: 1140 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 597 aa overlap). Long conserved hypothetical protein, equivalent to O53120|ML2678 OR MLCB1913.12 HYPOTHETICAL PROTEIN from Mycobacterium leprae (1000 aa), FASTA scores: opt: 760, E(): 7.1e-38, (50.2% identity in 1128 aa overlap); and middle part equivalent to Q9ZB40 72.2 KDA PROTEIN (FRAGMENT) from Mycobacterium leprae (644 aa), FASTA scores: opt: 1017, E(): 1.5e-65, (45.65% identity in 655 aa overlap). Also highly similar to Q98HG6|MLL2877 HYPOTHETICAL PROTEIN from Rhizobium loti (Mesorhizobium loti) (1119 aa), FASTA scores: opt: 1413, E(): 3.7e-77, (52.4% identity in 1148 aa overlap); and N-terminus shows similarity with other proteins e.g. Q9HUN8|PA4926 HYPOTHETICAL PROTEIN from Pseudomonas aeruginosa (311 aa), FASTA scores: opt: 278, E(): 3e-09, (29.95% identity in 284 aa overlap); and upstream ORF Q50652|YP69_MYCTU|Rv2569c|MT2645|MTCY227.32 CONSERVED HYPOTHETICAL PROTEIN from Mycobacterium tuberculosis (314 aa), FASTA scores: opt: 252, E(): 1.1e-07, (28.9% identity in 315 aa overlap). Equivalent to AAK46955 from Mycobacterium tuberculosis strain CDC1551 (1156 aa) but shorter 16 aa. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, Rv2566 exists as a single gene. In Mycobacterium bovis, a frameshift due to a 2 bp deletion (gc-*) splits Rv2566 into 2 parts, Mb2595 and Mb2596. Protein product from Mb2596 detected using SWATH mass spectrometry." /db_xref="InterPro:IPR018667" /db_xref="UniProtKB/TrEMBL:A0A1R3Y1K2" BEGIN 1 MLPLHRRDDG QGWASANWRL RRGRIVLLEG DSPAGLRLPL DSISWRPPRA SFDADPVAVR 61 STLPAEPHTD RAVVEDPETA PTTALVAEVR GGLVHIFLPP TDALEHFIDL VARVEAAATT 121 ANCPVVIEGY GPPPDPRLTS TTITPDPGVI EVNIAPTASF AEQRQQLETL YQQARLARLT 181 TEAFDVDGTH GGTGGGNHIT LGGVTPADSP LLRRPDLLVS LLTYWQRHPS LSYLFAGRFV 241 GTTSQAPRVD EGRAEALYEL EIAFAEILRL SPSSGGGRPQ PWVTDRALRH LLTDITGNTH 301 RAEFCIDKLY SPDSARGRLG LLELRGFEMP PHLHMAMVQS LLVRSLVAWF WDQPLRAPLI 361 RHGANLHGRY LLPHFLIHDI ADVAADLRAH GIAFETSWLD PFTEFRFPRI GTAVFDGIEI 421 ELRGAIEPWH TLGEEATAAG TARYVDSSVE RIQVRIIGAD RHRYVVTCNG YPMPLLATDN 481 PDIHVGGVRF KAWQPPSALH PTITVDGPLR FELIDIATAT SCGGCTYHVA HPGGRAYDEP 541 PVNAVEAEAR RARRFEATGF TPGKLDLSDI REKQARISTD IGAPGILDLR RVRTVQQ //