LOCUS SIU01025.1 563 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 ferredoxin- dependent sulfite reductase sira protein. ACCESSION LT708304-2439 PROTEIN_ID SIU01025.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="sira" /locus_tag="BQ2027_MB2412" /note="Mb2412, nirA, len: 563 aa. Equivalent to Rv2391, len: 563 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 563 aa overlap). Probable nirA, ferredoxin-dependant nitrite reductase (EC 1.7.7.1), similar to many nitrate reductases e.g. CAC33947|SCBAC1A6.26c Putative nitrite/sulphite reductase from Streptomyces coelicolor (565 aa), FASTA scores: opt: 2335, E(): 1.2e-137, (60.1% identity in 567 aa overlap); Q9RZD6|DRA0013 FERREDOXIN-NITRITE REDUCTASE from Deinococcus radiodurans (563 aa), FASTA scores: opt: 1141, E(): 2.2e-63, (39.6% identity in 533 aa overlap); Q59656|NIRA (D31732|PEENIRNRT_1) ferredoxin-dependant* NITRITE REDUCTASE (*: see citation below) from Plectonema boryanum (654 aa), FASTA scores: opt: 805, E(): 1.9e-42, (31.7% identity in 517 aa overlap); Q55366|NIRA|SLR0898 FERREDOXIN-NITRITE REDUCTASE from Synechocystis sp. strain PCC 6803 (502 aa), FASTA scores: opt: 799, E(): 3.7e-42, (32.3% identity in 517 aa overlap). Highly similar (only in N-terminal part because shortened protein (fragment) owing to an IS900 insertion) to Q9K541|NIRA NITRATE REDUCTASE (FRAGMENT) from Mycobacterium paratuberculosis (198 aa), FASTA scores: opt: 798, E(): 2.1e-42, (65.4% identity in 182 aa overlap). Protein product from Mb2412 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2412 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7TYP6" /db_xref="InterPro:IPR005117" /db_xref="InterPro:IPR006066" /db_xref="InterPro:IPR006067" /db_xref="InterPro:IPR036136" /db_xref="UniProtKB/Swiss-Prot:Q7TYP6" BEGIN 1 MSAKENPQMT TARPAKARNE GQWALGHREP LNANEELKKA GNPLDVRERI ENIYAKQGFD 61 SIDKTDLRGR FRWWGLYTQR EQGYDGTWTG DDNIDKLEAK YFMMRVRCDG GALSAAALRT 121 LGQISTEFAR DTADISDRQN VQYHWIEVEN VPEIWRRLDD VGLQTTEACG DCPRVVLGSP 181 LAGESLDEVL DPTWAIEEIV RRYIGKPDFA DLPRKYKTAI SGLQDVAHEI NDVAFIGVNH 241 PEHGPGLDLW VGGGLSTNPM LAQRVGAWVP LGEVPEVWAA VTSVFRDYGY RRLRAKARLK 301 FLIKDWGIAK FREVLETEYL KRPLIDGPAP EPVKHPIDHV GVQRLKNGLN AVGVAPIAGR 361 VSGTILTAVA DLMARAGSDR IRFTPYQKLV ILDIPDALLD DLIAGLDALG LQSRPSHWRR 421 NLMACSGIEF CKLSFAETRV RAQHLVPELE RRLEDINSQL DVPITVNING CPNSCARIQI 481 ADIGFKGQMI DDGHGGSVEG FQVHLGGHLG LDAGFGRKLR QHKVTSDELG DYIDRVVRNF 541 VKHRSEGERF AQWVIRAEED DLR //