LOCUS       SIU01010.1                71 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 MbtH-
            like NRPS chaperone => MbtH protein.
ACCESSION   LT708304-2424
PROTEIN_ID  SIU01010.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="mbtH"
                     /locus_tag="BQ2027_MB2398C"
                     /note="Mb2398c, mbtH, len: 71 aa. Equivalent to Rv2377c,
                     len: 71 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (98.6% identity in 71 aa overlap). Putative mbtH,
                     conserved protein with no function assigned (see first and
                     second citation), similar to hypothetical proteins or
                     proteins found in several gene clusters for biosynthesis
                     or transport of siderophores and other nonribosomally
                     synthesized peptides e.g. Q9Z388|SCE8.11c PUTATIVE SMALL
                     CONSERVED HYPOTHETICAL PROTEIN from Streptomyces
                     coelicolor (71 aa), FASTA scores: opt: 345, E(): 1.4e-19,
                     (68.2% identity in 66 aa overlap); Q9F8V3|CUMB COUY
                     PROTEIN (probably involved in the biosynthesis of
                     aminocoumarin antibiotic coumermycin A(1)) (see third
                     citation below) from Streptomyces rishiriensis (71 aa),
                     FASTA scores: opt: 329, E(): 2.2e-18, (63.2% identity in
                     68 aa overlap); Q9F5J2|SIM-CB MBTH-LIKE PROTEIN (probably
                     protein involved in the biosynthesis of aminocoumarin
                     antibiotic coumermycin A(1)) from Streptomyces
                     antibioticus (70 aa), FASTA scores: opt: 308, E():
                     8.4e-17, (65.6% identity in 64 aa overlap); Q9FB14
                     MBTH-LIKE PROTEIN (involved in the biosynthesis of the
                     antitumor drug bleomycin) (see fourth citation below) from
                     Streptomyces verticillus FASTA scores: opt: 220, E():
                     8.8e-10, (41.2% identity in 68 aa overlap); etc. Protein
                     product from Mb2398c detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb2398c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
                     /db_xref="InterPro:IPR005153"
                     /db_xref="InterPro:IPR037407"
                     /db_xref="InterPro:IPR038020"
                     /db_xref="UniProtKB/Swiss-Prot:P59965"
BEGIN
        1 MSTNPFDDDN GAFFVLVNDE DQHSLWPVFA DIPAGWRVVH GEASRAACLD YVEKNWTDLR
       61 PKSLRDAMAE D
//