LOCUS       SIT99954.1                78 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PUTATIVE
            ADA REGULATORY PROTEIN ALKAa [FIRST PART] (Regulatory
            protein of adaptative response) : Methylated-DNA--protein-
            cysteine methyltransferase (O-6-methylguanine-DNA alkyltransferase)
            protein.
ACCESSION   LT708304-1366
PROTEIN_ID  SIT99954.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="alkAa"
                     /locus_tag="BQ2027_MB1351C"
                     /note="Mb1351c, alkAa, len: 78 aa. Equivalent to the 5'
                     end of Rv1317c, len: 496 aa, from Mycobacterium
                     tuberculosis strain H37Rv, (98.7% identity in 78 aa
                     overlap). Putative alkA (alternate gene name: ada),
                     regulatory protein (EC 2.1.1.63), similar to
                     3MG2_ECOLI|P04395 dna-3-methyladenine glycosidase II from
                     Escherichia coli (282 aa), FASTA scores, opt: 437, E():
                     8.6e-22, (32.8% identity in 293 aa overlap), also similar
                     to other ada proteins e.g. ADA_SALTY|P26189 Salmonella
                     typhimurium (352 aa), FASTA scores: E(): 5.3e-08, (35.9%
                     identity in 156 aa overlap). Contains PS00041 Bacterial
                     regulatory proteins, araC family signature.
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, alkA exists as a single gene.
                     In Mycobacterium bovis, a single base transition (g-a)
                     introduces a premature stop codon that splits alkA into 2
                     parts, alkAa and alkAb. Mb1351c found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3XYZ1"
                     /db_xref="InterPro:IPR004026"
                     /db_xref="InterPro:IPR035451"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XYZ1"
BEGIN
        1 MHDDFERCYR AVQSKDARFD GWFVVAVLTT GVYCRPSCPV RPPFARNVRF LPTAAAAQGE
       61 GFRACKRCRP DASPGSPE
//