LOCUS       SIT99637.1               317 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            DIMETHYLADENOSINE TRANSFERASE KSGA (S-adenosylmethionine-
            6-N', N'-adenosyl(rRNA) dimethyltransferase) (16S rRNA
            dimethylase) (High level kasugamycin resistance protein
            ksgA) (Kasugamycin dimethyltransferase) protein.
ACCESSION   LT708304-1049
PROTEIN_ID  SIT99637.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="ksgA"
                     /locus_tag="BQ2027_MB1037"
                     /note="Mb1037, ksgA, len: 317 aa. Equivalent to Rv1010,
                     len: 317 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100% identity in 317 aa overlap). Probable ksgA,
                     dimethyladenosine transferase (EC 2.1.1.-), similar to
                     many e.g. KSGA_BACSU|P37468 dimethyladenosine transferase
                     from Bacillus subtilus (292 aa), FASTA scores: opt: 524,
                     E(): 1.5e-28, (37.2% identity in 274 aa overlap); similar
                     to Mycobacterium tuberculosis hypothetical protein Rv1988.
                     Contains PS01131 Ribosomal RNA adenine dimethylases
                     signature. Protein product from Mb1037 detected using
                     SWATH mass spectrometry. Mb1037 found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P66661"
                     /db_xref="InterPro:IPR001737"
                     /db_xref="InterPro:IPR011530"
                     /db_xref="InterPro:IPR020596"
                     /db_xref="InterPro:IPR020598"
                     /db_xref="InterPro:IPR023165"
                     /db_xref="InterPro:IPR029063"
                     /db_xref="UniProtKB/Swiss-Prot:P66661"
BEGIN
        1 MCCTSGCALT IRLLGRTEIR RLAKELDFRP RKSLGQNFVH DANTVRRVVA ASGVSRSDLV
       61 LEVGPGLGSL TLALLDRGAT VTAVEIDPLL ASRLQQTVAE HSHSEVHRLT VVNRDVLALR
      121 REDLAAAPTA VVANLPYNVA VPALLHLLVE FPSIRVVTVM VQAEVAERLA AEPGSKEYGV
      181 PSVKLRFFGR VRRCGMVSPT VFWPIPRVYS GLVRIDRYET SPWPTDDAFR RRVFELVDIA
      241 FAQRRKTSRN AFVQWAGSGS ESANRLLAAS IDPARRGETL SIDDFVRLLR RSGGSDEATS
      301 TGRDARAPDI SGHASAS
//