LOCUS       QJU56418.1               448 aa    PRT              BCT 18-MAY-2020
DEFINITION  Sphingomonas sp. AP4-R1 tRNA (N6-isopentenyl adenosine(
            37)-C2)-methylthiotransferase MiaB protein.
ACCESSION   CP053346-49
PROTEIN_ID  QJU56418.1
SOURCE      Sphingomonas sp. AP4-R1
  ORGANISM  Sphingomonas sp. AP4-R1
            Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Sphingomonas.
REFERENCE   1  (bases 1 to 5252057)
  AUTHORS   Heo,J., Kim,S.-J., Kim,J.-S., Hong,S.-B. and Kwon,S.-W.
  TITLE     Genome sequencing of strain KACC 21605
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5252057)
  AUTHORS   Heo,J., Kim,S.-J., Kim,J.-S., Hong,S.-B. and Kwon,S.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2020) Agricultural Mircrobiology Division,
            National Institute of Agricultural Sciences, 166
            Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365,
            South Korea
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: APR-2020
            Assembly Method        :: RS HGAP Assembly v. 3.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 99.0x
            Sequencing Technology  :: PacBio RSII
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/11/2020 21:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 4,788
            CDSs (total)                      :: 4,729
            Genes (coding)                    :: 4,613
            CDSs (with protein)               :: 4,613
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 56 of 116
            Pseudo Genes (incomplete)         :: 58 of 116
            Pseudo Genes (internal stop)      :: 26 of 116
            Pseudo Genes (multiple problems)  :: 22 of 116
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Sphingomonas sp. AP4-R1"
                     /mol_type="genomic DNA"
                     /strain="AP4-R1"
                     /host="Malus prunifolia (crab apple)"
                     /culture_collection="KACC:21605"
                     /db_xref="taxon:2735134"
                     /geo_loc_name="South Korea: Naju-si"
                     /collection_date="23-Oct-2019"
                     /collected_by="Jun Heo, Soon-Wo Kwon"
                     /identified_by="Jun Heo, Soon-Wo Kwon"
     protein         /gene="miaB"
                     /locus_tag="HL653_00245"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MTSPSTRPPS RPLPKTFSVK SFGCQMNAYD GARMAELLEE QGLVAVETDE ADLVLLNTCH
       61 IREKAAEKVY SDIGRLTKTA QGKGRAKPMI AVAGCVAQAE GAEIPRRAPA VDLVVGPQAY
      121 HRLPDLIAEA ASGKKALDTD MPAASKFDAL PKRRRQGPGA FLTVQEGCDK FCTYCVVPYT
      181 RGAEISRPWG QVVDEAKALV DAGAREITLL GQNVNAWTHE GRGLHDLIEE LARLPRLARI
      241 RYTTSHPNDM ADGLIAAHRD IDKLMPFLHL PVQAGSDRIL KAMNRSHDVA SYLKLIDRVR
      301 AVRPDIAISG DFIVGFPGET DAEFEETLSA VRAIGHAQAF SFKYSPRPGT PAATMGDAVP
      361 AAVMDERLQR LQALIAEQQY AFNRATLGKT CAVLIERDGK LPGQRLGKSP WLQSVLIDGG
      421 AIGDLVDVTI TQAGPNSLQG EAKVLAAA
//