LOCUS ETN69056.1 823 aa PRT CON 23-MAR-2015 DEFINITION Necator americanus EB module protein. ACCESSION KI669176-96 PROTEIN_ID ETN69056.1 SOURCE Necator americanus (New World hookworm) ORGANISM Necator americanus Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae; Bunostominae; Necator. REFERENCE 1 (bases 1 to 1237885) AUTHORS Mitreva,M. TITLE Draft genome of the hookworm Necator americanus JOURNAL Unpublished REFERENCE 2 (bases 1 to 1237885) AUTHORS Mitreva,M., Abubucker,S., Martin,J., Minx,P., Warren,C., Pepin,K.H., Palsikar,V.B., Zhang,X.W.E. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (17-APR-2013) The Genome Institute, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT Necator americanus is a roundworm that causes most of the human hookworm infections. N. americanus is a blood-feeding nematode infecting people in the rural areas of the tropics and subtropics causing an estimated disease burden of 22 million disability adjusted life years. The life cycle of the parasite begins when parasite eggs from an infected host are passed in the feces. In favorable environmental condition they hatch, and through 2 larval stages develop to the infective stage (L3). L3s penetrate human skin, migrate through the circulatory system and lung to finally reside in duodenum. The development continues and the adult stages attach to the intestinal mucosa and feed on blood. The strain being sequenced was obtained from the laboratory of Dr. Peter Hotez by Dr. Bin Zhan, originally isolated from an infected patient in Hunan province, China, and has been maintained in hamster since 1976.The adult worms were collected from intestines of hamsters infected subcutaneously with N. americanus L3 for 8 weeks. Worm isolation and DNA extraction was performed by Bin Zhan with QIAamp DNA mini kit according to manufacturers instructions (Qiagen). Jian et. al., 2003 Exp Parasitol. This assembly consists of fragments, 3kb and 8kb insert whole genome shotgun libraries. The sequences were generating on the Roch/454 platform and assembled using Newbler. To improve scaffolding, an in-house tool CIGA (Cdna tool for Improving Genome Assembly), was used to map 454 cDNA reads using blat to the genomic assembly to link genomic contigs based on cDNA evidence. Only joins confirmed by additional independent data typing were accepted. The repeat library was generated using Repeatmodeler (A.F.A. Smit, R. Hubley & P. Green http://repeatmasker.org). The Ribosomal RNA genes were identified using RNAmmer (Lagesen et. al., 2007 Nucleic Acids Res.) and transfer RNA's were identified with tRNAscan-SE (Lowe and Eddy, Nucleic Acids Res. 1997). Non-coding RNAs, such as microRNAs, were identified by sequence homology search of the Rfam database (Griffiths-Jones et. al., 2003 Nucleic Acids Res.). Repeats and predicted RNA's were then masked using RepeatMasker (A. Smit, R. Hubley & P. Green http://repeatmasker.org). Protein-coding genes were predicted using a combination of ab initio programs Snap (Korf, 2004 BCM Bioinformatics), Fgenesh (Salamov A., Solovyev V. 2000, Genome Res.) and Augustus (M. Stanke, et. al., 2008 Bioinformatics) and the annotation pipeline tool Maker (M. Yandell et. al., 2007 Genomic Research) which aligns mRNA, EST and protein information from same species or cross-species to aid in gene structure determination and modifications. A consensus gene set from the above prediction algorithms was generated, using a logical, hierarchical approach developed at the Genome institute. Gene product naming was determined by BER (http://ber.sourceforge.net). Our goal is to explore this WGS draft sequence of N. americanus to better define proteins involved in nematode parasitism that impact health and disease and are relevant to both host-parasite relationships and basic biological processes. For information regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. For specific questions regarding the N. americanus genome project contact Makedonka Mitreva (mmitreva@genome.wustl.edu) at Washington University School of Medicine. The National Human Genome Research Institute (NHGRI) of the National Institutes of Health (NIH) provided funds for this project. ##Genome-Assembly-Data-START## Finishing Goal :: High-Quality Draft Current Finishing Status :: High-Quality Draft Assembly Method :: Newbler v. MapAsmResearch-04/19/2010-patch- 08/17/2010 Assembly Name :: N_ americanus_v1 Genome Coverage :: 26.15x Sequencing Technology :: 454 ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Necator americanus" /mol_type="genomic DNA" /submitter_seqid="N_americanus-1.0_Cont9" /host="Homo sapiens" /db_xref="taxon:51031" /chromosome="Unknown" /lab_host="hamster" /country="China: Hunan Province" protein /locus_tag="NECAME_01166" /inference="protein motif:HMMPfam:IPR006149" /db_xref="InterPro:IPR006149" intron_pos 17:1 (1/21) intron_pos 54:0 (2/21) intron_pos 113:1 (3/21) intron_pos 150:2 (4/21) intron_pos 212:0 (5/21) intron_pos 231:1 (6/21) intron_pos 255:1 (7/21) intron_pos 305:1 (8/21) intron_pos 338:0 (9/21) intron_pos 374:1 (10/21) intron_pos 398:1 (11/21) intron_pos 434:2 (12/21) intron_pos 463:1 (13/21) intron_pos 513:0 (14/21) intron_pos 549:1 (15/21) intron_pos 573:0 (16/21) intron_pos 610:0 (17/21) intron_pos 652:0 (18/21) intron_pos 695:1 (19/21) intron_pos 733:2 (20/21) intron_pos 782:1 (21/21) BEGIN 1 MRTRCSSEKA PLFVLSVCDA NSLSGRMCMT VELYRTADNS SQFYECAPLA REEVVEYNLT 61 DKYLGVWNLR DCPENFDFDV SRQKCIERKT MRRQQAACAK NPASTGCQAP CSGAASIPTI 121 GSPCDWKEAA LHPDPKSNAY FVQCSPQSAG TPCGEWTRMA CAPNTVFSKP SSICVSMSVQ 181 SSACGSQREP VCNCAKTAGA TQCPGTSTCN KNVCCQQKDV IDNFIQHQAP YSACVQGLGC 241 CPVPVNQPKE AIQITLCPGS FSPPLGSCGS CPTGTSCSPA INACCPFATK SPPDLTFNVV 301 QLCPNGSPAL QSCSRGCPDG YSCYQGSCCP MSCPVGQTAL GFCSSGACSG GGSCYTPGGC 361 CCQETVKLPV CANGMWATYG MLEWRMLSDA ILSHRGTSPN ALWKQQYDLR FGTFMSGRAL 421 LSFTSLSKRN NLIRCSNGCA NCCPFGHSCV NGGCCPLPQC PAGGLALTMC GTRSCSVGNE 481 CVGGGCCPLP RCPSGLQATQ RCQRGIGCPR GHQCENGVCC AMPTCSAGLI AASVCGMGNS 541 CPMGFVCEVH PQKFILPQHY KFNNVDAQRG ECEEVVAKNR LHFAQMEAEL FNGAVEELNA 601 LQDLDVLLSE DVAFYRWNQC AQHKRTQSAN VLPIVLAHRV QRAQWERVVP KMPAKVLTLA 661 RNGCDQYGSP CTVLLSTARR KPIIAPVGNI TDAPLFFNVA TERRCVVNAT DVNFDPDSTC 721 LPNEKCINGE CRMKLWPGEY GCITDDECTS RCPNTYCEKK KSDKNVAQCQ CRDGQLLFGR 781 CFSQCPPGFH ESGAYCMHDD EDAFWADGDA QDRIKALLNA SPC //