LOCUS CAD77145.1 1072 aa PRT BCT 27-FEB-2015 DEFINITION Rhodopirellula baltica SH 1 acriflavine resistance protein B protein. ACCESSION BX294152-252 PROTEIN_ID CAD77145.1 SOURCE Rhodopirellula baltica SH 1 ORGANISM Rhodopirellula baltica SH 1 Bacteria; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rhodopirellula. REFERENCE 1 (bases 1 to 304250) AUTHORS Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R. TITLE Complete genome sequence of the marine planctomycete Pirellula sp. strain 1 JOURNAL Proc. Natl. Acad. Sci. U.S.A. 100(14), 8298-8303(2003). PUBMED 12835416 REFERENCE 2 (bases 1 to 304250) AUTHORS Kube M., Borzym K., Heitmann K., Klages S., Marquardt I., Lehrack S., Beck A., Pawlik R., Reinhardt R., Gloeckner F.O., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Rabus R., Schlesner H., Amann R. JOURNAL Submitted (21-JAN-2003) to the INSDC. Max Planck Institute for Molecular Genetics, proScience Ihnestrasse 73, D-14195 Berlin, Germany Max Planck Institute for Marine Microbiology Celsiusstrasse 1, D-28359 Bremen, Germany. COMMENT This project was carried out by *Max Planck Institute for Molecular Genetics, Berlin, Germany; *Max Planck Institute for Marine Microbiology, Bremen, Germany; in the fremework of the REGX-project, http://www.regx.de -------------- Genome Center Center: Max Planck Institute for Molecular Genetics Center code: MPIMG -------------- Summary Statistics Sequencing vector: pUC19; 100% of reads Chemistry: Dye-terminator Big Dye; 100% of reads Assembly program: Phrap; version 0.990329 Consensus quality: 7142841 bases at least Q40 Consensus quality: 7145138 bases at least Q30 Consensus quality: 7145484 bases at least Q20 Quality coverage: 8.03 -------------- This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistry, or coveredby high quality data (i.e., phred quality >= 30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; allregions were covered by at least one plasmid Sequence; assembly was additionally confirmed by long range pcr and cosmid end sequences. -------------- See http://www.micro-genomes.mpg.de/pirellula/ for more information including minimal tiling path from a set of 220 cosmids out of 908. See the misc_feature tag below for the boundaries of the MTP cosmids. -------------- Annotation Center: Max Planck Institute for Marine Microbiology Celsiusstrasse 1, D-28359 Bremen, Germany. Center Code: MPIMM Email: fog@mpi-bremen.de Phone: +49 (0)421 2028 938 Fax: +49 (0)421 2028 580 -------------- Three different programs (Glimmer, Critica and Orpheus) were used for ORF-prediction. A nonredundant list of ORFs was generated by suitable parsing of the results. Automated annotation was done with the software package Pedant Pro (http://www.biomax.de). All ORF predictions and annotations were manually corrected by considering all results of the different tools applied. See http://www.regx.de for more information and access to supplementary information. -------------- FEATURES Qualifiers source /organism="Rhodopirellula baltica SH 1" /strain="1" /mol_type="genomic DNA" /db_xref="taxon:243090" protein /transl_table=11 /gene="acrB" /locus_tag="RB11072" /function="drug transporters" /note="PMID: 8905231 PMID: 8407802 PMID: 7651136 best DB hits: BLAST: pir:F83335; RND multidrug efflux transporter MexF PA2494 [imported] -; E=0.0 pir:T30830; hypothetical protein mexF - Pseudomonas aeruginosa -----; E=0.0 pir:D83206; probable RND efflux transporter PA3522 [imported] -; E=0.0 COG: PA2494; COG0841 Cation/multidrug efflux pump; E=0.0 PFAM: PF00873; AcrB/AcrD/AcrF family; E=0" /db_xref="GOA:Q7UJT6" /db_xref="InterPro:IPR000731" /db_xref="InterPro:IPR001036" /db_xref="InterPro:IPR004764" /db_xref="InterPro:IPR027463" /db_xref="UniProtKB/TrEMBL:Q7UJT6" BEGIN 1 MDLTPTDRRS SCGSHDGGET MKFPHFFIER PIFASVLSFV IVLVGGIVYF TLPVSQYPSV 61 APPTVLVRAS YPGATPEVIA DTVATPIEQE MNGVDDMLYM ESSSSSDGTM QLTVTFKLGT 121 DLDDAQVLVQ NRVAIAESRL PEQVRQIGVT TTKQIPDMLM VVHLNSPDDS RDQLYISNYA 181 FLRVRDALMR LDGVGEIRIA GGNEYAMRIW LDIEKMTHVD LTAGEVVDAI QAQNIQVAAG 241 VIGQPPTDET GDFQLNVTTQ GRLIREDEFG DIIVKRGEDG RITRLRDVAR IELGAQDYSR 301 LSYLDGKSAI AVLVYQRPGT NAVDTAAEVK RVMSEMSEEF PQGIGYEIAY NPTDYVEDSI 361 AEVFETLFIT TIFVVLTVFL FLHGWRPTII PVIAIPISLI GTFAVMQFLG VTLNTLSLFG 421 LVLAIGIVVD DAIVVVENVE RLIAEGMTAR EATHKAMDEV GSALIATTLV LIAVFVPTVF 481 VPGISGQFYQ QFALTISIST AISTFVSLTL SPALCALLLR PKNAPKNRTG KAIDFLFGWF 541 FRLFNRTFDA TSNIYASIIG RLIKKTGFAM VLYVGLLILT GFSFSMVPTG FIPDQDQGYV 601 IVAINLPDGA SLARTDRVTR RVAEIGKEID GVAHAVGIAG LSGATFSIKP NAAVTFLPLE 661 DAKERAKRGR GVDAIVADMR REVAAINEAQ ILIIPPPPIR GIGRGGGFKM YVQDRSGAGL 721 DAMNEVTQTM LAKANQQPGV TQVFSNLRMD VPQVYADVDR TKAQMLDVPV NRVFEALQVY 781 LGSVYINDFN FLGRTYRVTA QAEPKFRDEA TDITKIRTRS DRGASIALGS VVNITQTAGP 841 DRLVRFNLFP AADLNGTTVP GFSTGQSLAT MEQLADQNLP PGFGYEWTEI AFQERQAGNT 901 IVFLFPLAVL FVFLALSAQY ESWLLPLAII LIVPLCLLFA LSGVWFRGMD NNVLTQIGFI 961 VLIGLACKNA ILIVEFAKAE EDAGKNRFDA AIAACRLRLR PILMTAFSFI LGVVPLLVAT 1021 GAGFEMRRVL GTAVFAGMLG VTVFGLFLTP VFYVMLRRFA KKKEPETTVD AV //