LOCUS CAD77027.1 395 aa PRT BCT 27-FEB-2015 DEFINITION Rhodopirellula baltica SH 1 muconate cycloisomerase I protein. ACCESSION BX294152-134 PROTEIN_ID CAD77027.1 SOURCE Rhodopirellula baltica SH 1 ORGANISM Rhodopirellula baltica SH 1 Bacteria; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rhodopirellula. REFERENCE 1 (bases 1 to 304250) AUTHORS Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R. TITLE Complete genome sequence of the marine planctomycete Pirellula sp. strain 1 JOURNAL Proc. Natl. Acad. Sci. U.S.A. 100(14), 8298-8303(2003). PUBMED 12835416 REFERENCE 2 (bases 1 to 304250) AUTHORS Kube M., Borzym K., Heitmann K., Klages S., Marquardt I., Lehrack S., Beck A., Pawlik R., Reinhardt R., Gloeckner F.O., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Rabus R., Schlesner H., Amann R. JOURNAL Submitted (21-JAN-2003) to the INSDC. Max Planck Institute for Molecular Genetics, proScience Ihnestrasse 73, D-14195 Berlin, Germany Max Planck Institute for Marine Microbiology Celsiusstrasse 1, D-28359 Bremen, Germany. COMMENT This project was carried out by *Max Planck Institute for Molecular Genetics, Berlin, Germany; *Max Planck Institute for Marine Microbiology, Bremen, Germany; in the fremework of the REGX-project, http://www.regx.de -------------- Genome Center Center: Max Planck Institute for Molecular Genetics Center code: MPIMG -------------- Summary Statistics Sequencing vector: pUC19; 100% of reads Chemistry: Dye-terminator Big Dye; 100% of reads Assembly program: Phrap; version 0.990329 Consensus quality: 7142841 bases at least Q40 Consensus quality: 7145138 bases at least Q30 Consensus quality: 7145484 bases at least Q20 Quality coverage: 8.03 -------------- This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistry, or coveredby high quality data (i.e., phred quality >= 30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; allregions were covered by at least one plasmid Sequence; assembly was additionally confirmed by long range pcr and cosmid end sequences. -------------- See http://www.micro-genomes.mpg.de/pirellula/ for more information including minimal tiling path from a set of 220 cosmids out of 908. See the misc_feature tag below for the boundaries of the MTP cosmids. -------------- Annotation Center: Max Planck Institute for Marine Microbiology Celsiusstrasse 1, D-28359 Bremen, Germany. Center Code: MPIMM Email: fog@mpi-bremen.de Phone: +49 (0)421 2028 938 Fax: +49 (0)421 2028 580 -------------- Three different programs (Glimmer, Critica and Orpheus) were used for ORF-prediction. A nonredundant list of ORFs was generated by suitable parsing of the results. Automated annotation was done with the software package Pedant Pro (http://www.biomax.de). All ORF predictions and annotations were manually corrected by considering all results of the different tools applied. See http://www.regx.de for more information and access to supplementary information. -------------- FEATURES Qualifiers source /organism="Rhodopirellula baltica SH 1" /strain="1" /mol_type="genomic DNA" /db_xref="taxon:243090" protein /transl_table=11 /locus_tag="RB10855" /function="metabolism of vitamins, cofactors, and prosthetic groups" /EC_number="5.5.1.1" /note="PMID: 8990288 best DB hits: BLAST: pir:H69855; chloromuconate cycloisomerase homolog ykfB - Bacillus; E=7e-22 pir:T51250; chloromuconate cycloisomerase homolog ykfB [imported] -; E=3e-19 ddbj:BAB06725.1; (AP001517) muconate cycloisomerase [Bacillus; E=3e-19 COG: BS_ykfB; COG1441 O-succinylbenzoate synthase and related enzymes; E=6e-23 PFAM: PF01188; Mandelate racemase / muconate; E=3.6e-27" /db_xref="GOA:Q7UK54" /db_xref="InterPro:IPR013342" /db_xref="InterPro:IPR029017" /db_xref="InterPro:IPR029065" /db_xref="InterPro:IPR034603" /db_xref="InterPro:IPR036849" /db_xref="UniProtKB/TrEMBL:Q7UK54" BEGIN 1 MHVTELTAFL VPLPLKREIK HASFTRTESI NLLIRCKLSN GTIGWGEGVP RPYVTGETPE 61 GCLQQLSQSN LHPALMQSAS NWPEVIAICD ALQFNNDSDD SRGCGSNSLR CAVELSLLDA 121 FGRHFGQPVS AVVPHVAEAA EIHVRQPRVR YSTTITSSGA KSERISALKM RAYGFAQCKI 181 KVGVDGQDDP SRVARIRRWI GGGVDLRLDA NEAWRPEEVA EKMRPLREFG VTCIEQPVAH 241 DQVDALASLR ESIGVPIMLD ESLTSRMDAE RAIELGTCDL FNLRLSKCGG FLSCLRLAAL 301 ARASGLGYQL GCHPGESGVL SAAGRHWASS VRDIRYLEGS YDRHLLTTLP TQEDITFRYG 361 GWAPALDSPG LGVTIREDWL RQNAKAERTF VVEAQ //