LOCUS       CAB5248184.1             286 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 3-hydroxybutyryl-
            coa dehydrogenase fadb2 (beta-hydroxybutyryl-coa dehydrogenase)
            (bhbd) protein.
ACCESSION   LT708304-483
PROTEIN_ID  CAB5248184.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fadB2"
                     /locus_tag="BQ2027_MB0477"
                     /note="Mb0477, fadB2, len: 286 aa. Equivalent to Rv0468,
                     len: 286 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 286 aa overlap). Probable fadB2,
                     3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157),
                     equivalent to CAC31978.1|AL583925 3-hydroxyacyl-CoA
                     dehydrogenase from Mycobacterium leprae (287 aa). Also
                     similar to many 3-hydroxybutyryl-CoA dehydrogenases e.g.
                     U32229|BJU32229_1 beta-hydroxybutyryl coenzyme A
                     dehydrogenase from Bradyrhizobium japonicum (293 aa),
                     FASTA scores: opt: 771, E(): 0, (45.7% identity in 282 aa
                     overlap). BELONGS TO THE 3-HYDROXYACYL-COA DEHYDROGENASE
                     FAMILY. Protein product from Mb0477 detected using shotgun
                     mass spectrometry and SWATH mass spectrometry. Mb0477
                     found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MSDAIQRVGV VGAGQMGSGI AEVSARAGVE VTVFEPAEAL ITAGRNRIVK SLERAVSAGK
       61 VTERERDRAL GLLTFTTDLN DLSDRQLVIE AVVEDEAVKS EIFAELDRVV TDPDAVLASN
      121 TSSIPIMKVA AATKQPQRVL GLHFFNPVPV LPLVELVRTL VTDEAAAART EEFASTVLGK
      181 QVVRCSDRSG FVVNALLVPY LLSAIRMVEA GFATVEDVDK AVVAGLSHPM GPLRLSDLVG
      241 LDTLKLIADK MFEEFKEPHY GPPPLLLRMV EAGQLGKKSG RGFYTY
//