LOCUS CAB5247976.1 210 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Allophanate hydrolase 2 subunit 1 (EC protein. ACCESSION LT708304-275 PROTEIN_ID CAB5247976.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0270C" /EC_number="3.5.1.54" /note="Mb0270c, -, len: 210 aa. Equivalent to Rv0264c, len: 210 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 210 aa overlap). Conserved hypothetical protein, equivalent to CAC32080.1|AL583926 conserved hypothetical protein from Mycobacterium leprae (222 aa). Also similar to others hypothetical proteins e.g. AL121596|SC51A_20 from Streptomyces coelicolor (252 aa), FASTA scores: opt: 420, E(): 2.7e-20, (41.7% identity in 204 aa overlap); P75744|YBGJ_ECOLI HYPOTHETICAL 23.9 KD PROTEIN from Escherichia coli (218 aa), FASTA scores: E(): 2.1e-14, (35.7% identity in 182 aa overlap); YH31_HAEIN|P44299|hi173 hypothetical protein from Haemophilus influenzae (213 aa), FASTA scores: opt: 252, E(): 8.3e-10, (31.1% identity in 183 aa overlap). Protein product from Mb0270c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0270c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." BEGIN 1 MDAALACTVL DYGDHALMLQ CDSTADAMAW TDALRAAALP GVVDIVAASR TVLVKLDAPR 61 YQGVTRQRLR RLRVTPEAVA AADHRCDLVI DVVYDGPDLA EVARCTGLTT AAVINAHTAT 121 GWRAGFSGSA PGFAYLIDGD PSLRVPRRPE RRTSMPPGSV ALADGFSAIY PSQAPSDWQI 181 IGHTDAVLWD VDRPQPALLT PGMWVQFRAA //