LOCUS       CAB5247926.1             537 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            FATTY-ACID-COA LIGASE FADD4 (FATTY-ACID-COA SYNTHETASE)
            (FATTY-ACID-COA SYNTHASE) protein.
ACCESSION   LT708304-225
PROTEIN_ID  CAB5247926.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fadD4"
                     /locus_tag="BQ2027_MB0220"
                     /note="Mb0220, fadD4, len: 537 aa. Equivalent to Rv0214,
                     len: 537 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.8% identity in 537 aa overlap). Probable fadD4,
                     fatty-acid-CoA synthetase (EC 6.2.1.-), similar to many
                     e.g. 4CL_PINTA|P41636 4-coumarate--coa ligase (EC
                     6.2.1.12) (537 aa), FASTA scores: opt: 622, E(): 1e-31,
                     (30.0% identity in 514 aa overlap). Also similar to others
                     from Mycobacterium tuberculosis e.g. MTCY6A4.14 FASTA
                     score: (30.7% identity in 501 aa overlap); MTCY493_27,
                     MTCY07A7_11, MTCI28_6. Contains PS00455 putative
                     AMP-binding domain signature. Protein product from Mb0220
                     detected using SWATH mass spectrometry. Mb0220 found to be
                     expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
BEGIN
        1 MPRGELYKRF RLVMGGIAPC GSGRRAATYP RRMQIRPYIA ADKPAVILYP SGTVISFDEL
       61 EARANRLAHW FRQAGLREDD VVAILMENNE HVHAVMWAAR RSGLYYVPIN THLTASEAAY
      121 IVDNSGAKAI VGSAALRETC HGLAEHLPGG LPDLLMLAGG GLVGWMTYPE CVADQPDTPI
      181 EDEREGDLLQ YSSGTTGRPK GIKRELPHVS PDAAPGMMPA LLDFWMDADS VYLSPAPMYH
      241 TAPSVWTMSA LAAGVTTVVM EKFDAEGALD AIQRYRVTHA QFVPAMFVRM LKLPEAVRNS
      301 YDMSSLRRVI HAAAPCPVQI KEQMIHWWGP IIDEYYASSE ASGSTLITAE DWLTHPGSVG
      361 KPIQGGVHIV GADGSELPPN QPGEIYFEGG YPFEYLNDPA KTAASRNKHG WVTVGDVGYL
      421 DDDGYLFLTG RRHHMIISGG VNIYPQEAEN LLVAHPKVLD AAVFGVPDDE MGQRVMAAVQ
      481 TVDSADANDQ FAGELLAWLR DRLSHFKCPR SIAFEPQLPR TDTGKLYKSG LVEKYSV
//