LOCUS       CAB5247793.1             492 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 possible
            formate hydrogenase hyce (fhl) protein.
ACCESSION   LT708304-92
PROTEIN_ID  CAB5247793.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="hycE"
                     /locus_tag="BQ2027_MB0090"
                     /note="Mb0090, hycE, len: 492 aa. Equivalent to Rv0087,
                     len: 492 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.8% identity in 492 aa overlap). Possible hycE
                     (alternate gene name: hevE), formate hydrogenlyase (EC
                     1.-.-.-), similar to others e.g. HYCE_ECOLI|P16431 formate
                     hydrogenlyase subunit 5 from Escherichia coli (569 aa),
                     FASTA scores: opt: 680, E(): 1.8e-38, (31.2% identity in
                     449 aa overlap); NP_457243.1|NC_003198 formate
                     hydrogenlyase subunit 5 from Salmonella enterica subsp.
                     enterica serovar Typhi (569 aa); NP_275541.1|NC_000916
                     formate hydrogenlyase subunit 5 from Methanothermobacter
                     thermautotrophicus (370 aa); etc. Also some similarity
                     with NUOD_ECOLI|P33600 NADH dehydrogenase I chain D from
                     Escherichia coli (407 aa), FASTA scores: opt: 245, E():
                     8.9e-10, (24.5% identity in 368 aa overlap). BELONGS TO
                     THE COMPLEX I 49 kDa SUBUNIT FAMILY. Protein product from
                     Mb0090 detected using SWATH mass spectrometry."
BEGIN
        1 MMSASWLRHR VSERGLIATA EQLWADSFRL ALVAAHDDGD SLRVVYLFLA GYPDRRVELE
       61 YVVPADNPEI RSLAYLSFPA GRFEREMADL YGIRPVGHPK PRRLVRHAHW PDWHPMRTDA
      121 GPAPEFTDTG AFPFLAVEGP GVYEIPVGPV HAGLIEPGHF RFSVAGETIV RLKARLWFVH
      181 RGIEKLFHGR PATAAVDLAE RISGDTSAAH ALAHSLAIED ALGIELPHEI HRLRALIVEL
      241 ERLYNHAADL GALANDVGYS LANAHAQRIR ENLLRRNAAV TGHRLLRGAI RAGGVALRAL
      301 PDTDELAALA VDLAEVATLT LANSVVYDRF AGTAVLHPDD ASALGCLGYV ARASGLRSDA
      361 RVEHPTIVLP ITEIGAPDGD VLARYTVRRD EFAASAALAQ HIVESHTGPI EYAATLHPVG
      421 APSSGIGIVE GWRGTIVHRV EIDVDGRITR AKVVDPSWFN WPALPVAMAD TIVPDFPLAN
      481 KSFNQSYAGN DL
//