LOCUS CAB40665.1 55 aa PRT VRL 18-APR-2005 DEFINITION Human adenovirus 5 protein ( Adenovirus type 5 left 32% of the genome (coordinates 0% to 32.39% as measured by ). ). ACCESSION X02996-3 PROTEIN_ID CAB40665.1 SOURCE Human adenovirus 5 ORGANISM Human adenovirus 5 Viruses; Adenoviridae; Mastadenovirus. REFERENCE 1 (bases 1 to 194) AUTHORS Steenbergh P.H., Maat J., Van ormondt H., Sussenbach J.S. TITLE The nucleotide sequence at the termini of adenovirus type 5 DNA JOURNAL Nucleic Acids Res. 4(12), 4371-4389(1977). PUBMED 600799 REFERENCE 2 (bases 1 to 1574) AUTHORS Van ormondt H., Maat J., De Waard A., van der Eb A.J. TITLE The nucleotide sequence of the transforming HpaI-E fragment of adenovirus type 5 DNA. JOURNAL Gene 4(4), 309-328(1978). PUBMED 744489 REFERENCE 3 (bases 1575 to 2809) AUTHORS Maat J., Van ormondt H. TITLE The nucleotide sequence of the transforming hindiii-g fragment of adenovirus type 5 dna JOURNAL Gene 6(1), 75-90(1979). PUBMED 478299 REFERENCE 4 AUTHORS Perricaudet M., Akusjarvi G., Virtanen A., Pettersson U. TITLE Structure of two spliced mRNAs from the transforming region of human subgroup C adenoviruses JOURNAL Nature 281(5733), 694-696(1979). PUBMED 551290 REFERENCE 5 (bases 10524 to 10696) AUTHORS Thimmappaya B., Jones N., Shenk T. TITLE A mutation which alters initiation of transcription by RNA polymerase III on the Ad5 chromosome JOURNAL Cell 18(4), 947-954(1979). PUBMED 519773 REFERENCE 6 (bases 10555 to 10733) AUTHORS Fowlkes D.M., Shenk T. TITLE Transcriptional control regions of the adenovirus VAI RNA gene JOURNAL Cell 22(2 Pt 2), 405-413(1980). PUBMED 7448868 REFERENCE 7 (bases 2804 to 4125) AUTHORS Maat J., Van Beveren C.P., Van ormondt H. TITLE The nucleotide sequence of adenovirus type 5 early region E1: the region between map positions 8.0 (HindIII site) and 11.8 (SmaI site) JOURNAL Gene 10(1), 27-38(1980). PUBMED 6250944 REFERENCE 8 (bases 1 to 4125) AUTHORS Van ormondt H., Maat J., Van Beveren C.P. TITLE The nucleotide sequence of the transforming early region E1 of adenovirus type 5 DNA. JOURNAL Gene 11(3-4), 299-309(1980). PUBMED 6260576 REFERENCE 9 AUTHORS Perricaudet M., Le Moullec J.M., Pettersson U. TITLE Predicted structure of two adenovirus tumor antigens JOURNAL Proc. Natl. Acad. Sci. U.S.A. 77(7), 3778-3782(1980). PUBMED 6253988 REFERENCE 10 (bases 1 to 6246) AUTHORS Broker T.R. TITLE Appendix d: nucleotide sequences, transcription and translation analyses, and restriction endonuclease cleavage maps of group-c human adenoviruses JOURNAL (in) Tooze J. (Eds.); DNA TUMOR VIRUSES:937-1002; Cold Spring Harbor Laboratory (1980) REFERENCE 11 (bases 1653 to 4043) AUTHORS Bos J.L., Polder L.J., Bernards R., Schrier P.I., Van den elsen P.J., van der Eb A.J., Van ormondt H. TITLE The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets JOURNAL Cell 27(1 Pt 2), 121-131(1981). PUBMED 7326748 REFERENCE 12 (bases 4001 to 6246) AUTHORS Van Beveren C.P., Maat J., Dekker B.M., Van ormondt H. TITLE The nucleotide sequence of the gene for protein IVa2 and of the 5' leader segment of the major late mRNAs of adenovirus type 5 JOURNAL Gene 16(1-3), 179-189(1981). PUBMED 7343420 REFERENCE 13 (bases 1 to 66) AUTHORS Nagata K., Guggenheimer R.A., Hurwitz J. TITLE Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80(20), 6177-6181(1983). PUBMED 6336326 REFERENCE 14 (bases 325 to 604) AUTHORS Hearing P., Shenk T. TITLE Functional analysis of the nucleotide sequence surrounding the cap site for adenovirus type 5 region E1A messenger RNAs JOURNAL J. Mol. Biol. 167(4), 809-822(1983). PUBMED 6876165 REMARK deletion mutants REFERENCE 15 AUTHORS Downey J.F., Evelegh C.M., Branton P.E., Bayley S.T. TITLE Peptide maps and N-terminal sequences of polypeptides from early region 1A of human adenovirus 5 JOURNAL J. Virol. 50(1), 30-37(1984). PUBMED 6699947 REMARK sites; cds start for E1a proteins REFERENCE 16 (bases 6242 to 11570) AUTHORS Dekker B.M.M., Van ormondt H. TITLE The nucleotide sequence of fragment HindIII-C of human adenovirus type 5 DNA (map positions 17.1-31.7) JOURNAL Gene 27(1), 115-120(1984). PUBMED 6325298 COMMENT sites; splice sites in E1a 13S mRNA sites; splice sites and termini for E1b mRNAS Notes on the presentation of ADENO in the EMBL data library: The genetic map of Adeno is customarily presented from left to right, the 0% position being left and the 100% position being right. The two strands of Adeno are normally represented like this: r-strand: 3'------------------5' l-strand: 5'------------------3' ^ ^ 0% 100% This often causes confusion because the generally accepted way to represent DNA molecules is: 5'------------------3' 3'------------------5' Here Adeno virus sequences are always given in 5' to 3' direction and the sequence of the l-strand is displayed irrespective of the direction the viral transcription takes. This sequence corresponds to bases 1 to 11560 of , which serve as some basis for the annotation of sites. the differences between and are too many to report herein, however a printout of those is available upon request from genbank. the map coordinates in the sites presume 360 bases per map unit. although there are approximately 115 sequence differences between the two strains over this region, no site difference exceeds 0.02% by this calculation. the sequence represents the early mrna transcripts e1a and e1b and the intermediate mrna transcript ix, all of which are transcribed rightwardly off the r-strand; the iva2 and e2b mrnas which are transcribed leftwardly off the l-strand ((indicated by "(c)" and "comp strand" below)); and the 5' end of the 28 kb major late mrna. the cap sites and possible promoter sequences for these are summarized in the following table: mrna cap site possible promoter region ------ ---------- -------------------------- e1a 499 tatttata at bases 468-475 [6] e1b 1702 tatataa at bases 1672-1678[6] ix 3582 tatataa at bases 3551-3557 [6] iva2 5838 +/- 2 (c) tacaaa at bases 5979-5974 on the comp strand [10] major 6049 tataaaa at bases 6018-6024 [10] as with ad2, not all the transcripts from this region have been characterized at the sequence level. the nine proteins given in features table below are not the only possible gene products (see the main adenovirus 2 entry). large amounts of small rnas are produced from the vai and vaii genes late in development for unknown reasons. [5] and [6] demonstrate that vai gene activity influences vaii expression; that the 5' flank affects the start site of the rna but that an intragenic promoter (bases 10626 to 10690 below) determines whether the rna is actually produced; and that there is striking similarity between this rna and trna. FEATURES Qualifiers source /organism="Human adenovirus 5" /mol_type="genomic DNA" /db_xref="taxon:28285" protein /note="putative E1a protein from 9s mrna (splice sites determined for ad2)" /db_xref="GOA:P03255" /db_xref="InterPro:IPR014410" /db_xref="PDB:2KJE" /db_xref="PDB:2R7G" /db_xref="UniProtKB/Swiss-Prot:P03255" intron_pos 27:0 (1/1) BEGIN 1 MRHIICHGGV ITEEMAASLL DQLIEEVLCL NLSLSPSQNR SLQDLPAVLK WRLLS //