LOCUS CAA23425.1 338 aa PRT SYN 17-MAY-1995 DEFINITION synthetic construct protein ( Artificial cloning vehicle pBR327, derived from pBR322. The sequence was not resequenced but deduced from the pBR322 sequence. Contains the reading frames for ampicillin resistance (Apr) and tetracycline resistance (Tcr) and an origin of replication. ). ACCESSION V00083-1 PROTEIN_ID CAA23425.1 SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. REFERENCE 1 (bases 1 to 3273) AUTHORS Soberon X., Covarrubias L., Bolivar F. TITLE Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325 JOURNAL Gene 9(3-4), 287-305(1980). PUBMED 6248430 FEATURES Qualifiers source /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" protein /transl_table=11 /note="tetracycline resistance" BEGIN 1 MRTRSRSTVR PLWPPPSPAR FATWSHYRLR DHGDHTRPVD PLRRTHRGRH HRRHRCGCWR 61 LYRRHHRWGR SGSPLRAHER LFRRGYGGRP VAGGLLGAIS LHAPFLAAAV LNGLNLLLGC 121 FLMQESHKGE RRPMPLRAFN PVSSFRWARG MTIVAALMTV FFIMQLVGQV PAALWVIFGE 181 DRFRWSATMI GLSLAVFGIL HALAQAFVTG PATKRFGEKQ AIIAGMAADA LGYVLLAFAT 241 RGWMAFPIMI LLASGGIGMP ALQAMLSRQV DDDHQGQLQG SLAALTSLTS ITGPLIVTAI 301 YAASASTWNG LAWIVGAALY LVCLPALRRG AWSRATST //