LOCUS       BAL40669.1               547 aa    PRT              BCT 04-JUN-2019
DEFINITION  Escherichia coli str. K-12 substr. MDS42 predicted metal
            dependent hydrolase protein.
ACCESSION   AP012306-3349
PROTEIN_ID  BAL40669.1
SOURCE      Escherichia coli str. K-12 substr. MDS42
  ORGANISM  Escherichia coli str. K-12 substr. MDS42
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3976195)
  AUTHORS   Ying,B.W., Seno,S., Suzuki,S. and Yomo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Shigeto Seno
            Osaka University, Graduate School of Information Science and
            Technology; Yamadaoka 1-5, Suita, Osaka 565-0871, Japan
REFERENCE   2  
  AUTHORS   Fredens,J., Wang,K., de la Torre,D., Funke,L.F.H., Robertson,W.E.,
            Christova,Y., Chia,T., Schmied,W.H., Dunkelmann,D.L., Beranek,V.,
            Uttamapinant,C., Llamazares,A.G., Elliott,T.S. and Chin,J.W.
  TITLE     Total synthesis of Escherichia coli with a recoded genome
  JOURNAL   Nature 569, 514-518 (2019)
  REMARK    DOI:10.1038/s41586-019-1192-5
COMMENT     MDS42 is a genome reduced strain constructed from MG1655 by Posfai
            et al (originally reported in Science 2006), and is commercially
            available from Scarab Genomics (the founder is Dr. Frederick R.
            Blattner). We re-confirmed the whole genome sequence of MDS42 by
            the next generation resequencing technology based on SOLiD3
            (Applied Biosystems)
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: Bioscope v. 1.3
            Genome Coverage       :: 300x
            Sequencing Technology :: SOLiD 3
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /db_xref="taxon:1110693"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli str. K-12 substr. MDS42"
                     /strain="K-12"
                     /sub_strain="MDS42"
     protein         /gene="eptA"
                     /gene_synonym="ECK4107"
                     /gene_synonym="JW5730"
                     /gene_synonym="b4114"
                     /locus_tag="ECMDS42_3553"
                     /transl_table=11
BEGIN
        1 MLKRLLKRPS LNLLAWLLLA AFYISICLNI AFFKQVLQAL PLDSLHNVLV FLSMPVVAFS
       61 VINIVLTLSS FLWLNRPLAC LFILVGAAAQ YFIMTYGIVI DRSMIANIID TTPAESYALM
      121 TPQMLLTLGF SGVLAALIAC WIKIKPATSR LRSVLFRGAN ILVSVLLILL VAALFYKDYA
      181 SLFRNNKELV KSLSPSNSIV ASWSWYSHQR LANLPLVRIG EDAHRNPLMQ NEKRKNLTIL
      241 IVGETSRAEN FSLNGYPRET NPRLAKDNVV YFPNTASCGT ATAVSVPCMF SDMPREHYKE
      301 ELAQHQEGVL DIIQRAGINV LWNDNDGGCK GACDRVPHQN VTALNLPDQC INGECYDEVL
      361 FHGLEEYINN LQGDGVIVLH TIGSHGPTYY NRYPPQFRKF TPTCDTNEIQ TCTKEQLVNT
      421 YDNTLVYVDY IVDKAINLLK EHQDKFTTSL VYLSDHGESL GENGIYLHGL PYAIAPDSQK
      481 QVPMLLWLSE DYQKRYQVDQ NCLQKQAQTQ HYSQDNLFST LLGLTGVETK YYQAADDILQ
      541 TCRRVSE
//