LOCUS       BAL40565.1               441 aa    PRT              BCT 04-JUN-2019
DEFINITION  Escherichia coli str. K-12 substr. MDS42 fused DNA-binding
            response regulator in two-component regulatory system
            with ZraS protein.
ACCESSION   AP012306-3245
PROTEIN_ID  BAL40565.1
SOURCE      Escherichia coli str. K-12 substr. MDS42
  ORGANISM  Escherichia coli str. K-12 substr. MDS42
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3976195)
  AUTHORS   Ying,B.W., Seno,S., Suzuki,S. and Yomo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Shigeto Seno
            Osaka University, Graduate School of Information Science and
            Technology; Yamadaoka 1-5, Suita, Osaka 565-0871, Japan
REFERENCE   2  
  AUTHORS   Fredens,J., Wang,K., de la Torre,D., Funke,L.F.H., Robertson,W.E.,
            Christova,Y., Chia,T., Schmied,W.H., Dunkelmann,D.L., Beranek,V.,
            Uttamapinant,C., Llamazares,A.G., Elliott,T.S. and Chin,J.W.
  TITLE     Total synthesis of Escherichia coli with a recoded genome
  JOURNAL   Nature 569, 514-518 (2019)
  REMARK    DOI:10.1038/s41586-019-1192-5
COMMENT     MDS42 is a genome reduced strain constructed from MG1655 by Posfai
            et al (originally reported in Science 2006), and is commercially
            available from Scarab Genomics (the founder is Dr. Frederick R.
            Blattner). We re-confirmed the whole genome sequence of MDS42 by
            the next generation resequencing technology based on SOLiD3
            (Applied Biosystems)
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: Bioscope v. 1.3
            Genome Coverage       :: 300x
            Sequencing Technology :: SOLiD 3
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /db_xref="taxon:1110693"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli str. K-12 substr. MDS42"
                     /strain="K-12"
                     /sub_strain="MDS42"
     protein         /gene="zraR"
                     /gene_synonym="ECK3996"
                     /gene_synonym="JW3968"
                     /gene_synonym="b4004"
                     /locus_tag="ECMDS42_3442"
                     /transl_table=11
BEGIN
        1 MTHDNIDILV VDDDISHCTI LQALLRGWGY NVALANSGRQ ALEQVREQVF DLVLCDVRMA
       61 EMDGIATLKE IKALNPAIPV LIMTAYSSVE TAVEALKTGA LDYLIKPLDF DNLQATLEKA
      121 LAHTHSIDAE TPAVTASQFG MVGKSPAMQH LLSEIALVAP SEATVLIHGD SGTGKELVAR
      181 AIHASSARSE KPLVTLNCAA LNESLLESEL FGHEKGAFTG ADKRREGRFV EADGGTLFLD
      241 EIGDISPMMQ VRLLRAIQER EVQRVGSNQI ISVDVRLIAA THRDLAAEVN AGRFRQDLYY
      301 RLNVVAIEVP SLRQRREDIP LLAGHFLQRF AERNRKAVKG FTPQAMDLLI HYDWPGNIRE
      361 LENAVERAVV LLTGEYISER ELPLAIASTP IPLGQSQDIQ PLVEVEKEVI LAALEKTGGN
      421 KTEAARQLGI TRKTLLAKLS R
//