LOCUS       BAL40555.1               631 aa    PRT              BCT 04-JUN-2019
DEFINITION  Escherichia coli str. K-12 substr. MDS42 thiamin (pyrimidine
            moiety) biosynthesis protein protein.
ACCESSION   AP012306-3235
PROTEIN_ID  BAL40555.1
SOURCE      Escherichia coli str. K-12 substr. MDS42
  ORGANISM  Escherichia coli str. K-12 substr. MDS42
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3976195)
  AUTHORS   Ying,B.W., Seno,S., Suzuki,S. and Yomo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Shigeto Seno
            Osaka University, Graduate School of Information Science and
            Technology; Yamadaoka 1-5, Suita, Osaka 565-0871, Japan
REFERENCE   2  
  AUTHORS   Fredens,J., Wang,K., de la Torre,D., Funke,L.F.H., Robertson,W.E.,
            Christova,Y., Chia,T., Schmied,W.H., Dunkelmann,D.L., Beranek,V.,
            Uttamapinant,C., Llamazares,A.G., Elliott,T.S. and Chin,J.W.
  TITLE     Total synthesis of Escherichia coli with a recoded genome
  JOURNAL   Nature 569, 514-518 (2019)
  REMARK    DOI:10.1038/s41586-019-1192-5
COMMENT     MDS42 is a genome reduced strain constructed from MG1655 by Posfai
            et al (originally reported in Science 2006), and is commercially
            available from Scarab Genomics (the founder is Dr. Frederick R.
            Blattner). We re-confirmed the whole genome sequence of MDS42 by
            the next generation resequencing technology based on SOLiD3
            (Applied Biosystems)
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: Bioscope v. 1.3
            Genome Coverage       :: 300x
            Sequencing Technology :: SOLiD 3
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /db_xref="taxon:1110693"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli str. K-12 substr. MDS42"
                     /strain="K-12"
                     /sub_strain="MDS42"
     protein         /gene="thiC"
                     /gene_synonym="ECK3986"
                     /gene_synonym="JW3958"
                     /gene_synonym="b3994"
                     /locus_tag="ECMDS42_3432"
                     /transl_table=11
BEGIN
        1 MSATKLTRRE QRARAQHFID TLEGTAFPNS KRIYITGTHP GVRVPMREIQ LSPTLIGGSK
       61 EQPQYEENEA IPVYDTSGPY GDPQIAINVQ QGLAKLRQPW IDARGDTEEL TVRSSDYTKA
      121 RLADDGLDEL RFSGVLTPKR AKAGRRVTQL HYARQGIITP EMEFIAIREN MGRERIRSEV
      181 LRHQHPGMSF GAHLPENITA EFVRDEVAAG RAIIPANINH PESEPMIIGR NFLVKVNANI
      241 GNSAVTSSIE EEVEKLVWST RWGADTVMDL STGRYIHETR EWILRNSPVP IGTVPIYQAL
      301 EKVNGIAEDL TWEAFRDTLL EQAEQGVDYF TIHAGVLLRY VPMTAKRLTG IVSRGGSIMA
      361 KWCLSHHQEN FLYQHFREIC EICAAYDVSL SLGDGLRPGS IQDANDEAQF AELHTLGELT
      421 KIAWEYDVQV MIEGPGHVPM QMIRRNMTEE LEHCHEAPFY TLGPLTTDIA PGYDHFTSGI
      481 GAAMIGWFGC AMLCYVTPKE HLGLPNKEDV KQGLITYKIA AHAADLAKGH PGAQIRDNAM
      541 SKARFEFRWE DQFNLALDPF TARAYHDETL PQESGKVAHF CSMCGPKFCS MKISQEVRDY
      601 AATQTIEMGM ADMSENFRAR GGEIYLRKEE A
//