LOCUS       BAL40030.1               694 aa    PRT              BCT 04-JUN-2019
DEFINITION  Escherichia coli str. K-12 substr. MDS42 4-alpha-glucanotransferase 
            (amylomaltase) protein.
ACCESSION   AP012306-2710
PROTEIN_ID  BAL40030.1
SOURCE      Escherichia coli str. K-12 substr. MDS42
  ORGANISM  Escherichia coli str. K-12 substr. MDS42
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3976195)
  AUTHORS   Ying,B.W., Seno,S., Suzuki,S. and Yomo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Shigeto Seno
            Osaka University, Graduate School of Information Science and
            Technology; Yamadaoka 1-5, Suita, Osaka 565-0871, Japan
REFERENCE   2  
  AUTHORS   Fredens,J., Wang,K., de la Torre,D., Funke,L.F.H., Robertson,W.E.,
            Christova,Y., Chia,T., Schmied,W.H., Dunkelmann,D.L., Beranek,V.,
            Uttamapinant,C., Llamazares,A.G., Elliott,T.S. and Chin,J.W.
  TITLE     Total synthesis of Escherichia coli with a recoded genome
  JOURNAL   Nature 569, 514-518 (2019)
  REMARK    DOI:10.1038/s41586-019-1192-5
COMMENT     MDS42 is a genome reduced strain constructed from MG1655 by Posfai
            et al (originally reported in Science 2006), and is commercially
            available from Scarab Genomics (the founder is Dr. Frederick R.
            Blattner). We re-confirmed the whole genome sequence of MDS42 by
            the next generation resequencing technology based on SOLiD3
            (Applied Biosystems)
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: Bioscope v. 1.3
            Genome Coverage       :: 300x
            Sequencing Technology :: SOLiD 3
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /db_xref="taxon:1110693"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli str. K-12 substr. MDS42"
                     /strain="K-12"
                     /sub_strain="MDS42"
     protein         /gene="malQ"
                     /gene_synonym="ECK3403"
                     /gene_synonym="JW3379"
                     /gene_synonym="b3416"
                     /locus_tag="ECMDS42_2862"
                     /transl_table=11
BEGIN
        1 MESKRLDNAA LAAGISPNYI NAHGKPQSIS AETKRRLLDA MHQRTATKVA VTPVPNVMVY
       61 TSGKKMPMVV EGSGEYSWLL TTEEGTQYKG HVTGGKAFNL PTKLPEGYHT LTLTQDDQRA
      121 HCRVIVAPKR CYEPQALLNK QKLWGACVQL YTLRSEKNWG IGDFGDLKAM LVDVAKRGGS
      181 FIGLNPIHAL YPANPESASP YSPSSRRWLN VIYIDVNAVE DFHLSEEAQA WWQLPTTQQT
      241 LQQARDADWV DYSTVTALKM TALRMAWKGF AQRDDEQMAA FRQFVAEQGD SLFWQAAFDA
      301 LHAQQVKEDE MRWGWPAWPE MYQNVDSPEV RQFCEEHRDD VDFYLWLQWL AYSQFAACWE
      361 ISQGYEMPIG LYRDLAVGVA EGGAETWCDR ELYCLKASVG APPDILGPLG QNWGLPPMDP
      421 HIITARAYEP FIELLRANMQ NCGALRIDHV MSMLRLWWIP YGETADQGAY VHYPVDDLLS
      481 ILALESKRHR CMVIGEDLGT VPVEIVGKLR SSGVYSYKVL YFENDHEKTF RAPKAYPEQS
      541 MAVAATHDLP TLRGYWECGD LTLGKTLGLY PDEVVLRGLY QDRELAKQGL LDALHKYGCL
      601 PKRAGHKASL MSMTPTLNRG LQRYIADSNS ALLGLQPEDW LDMAEPVNIP GTSYQYKNWR
      661 RKLSATLESM FADDGVNKLL KDLDRRRRAA AKKK
//