LOCUS       BAL38886.1               415 aa    PRT              BCT 04-JUN-2019
DEFINITION  Escherichia coli str. K-12 substr. MDS42 multidrug efflux
            system, subunit A protein.
ACCESSION   AP012306-1566
PROTEIN_ID  BAL38886.1
SOURCE      Escherichia coli str. K-12 substr. MDS42
  ORGANISM  Escherichia coli str. K-12 substr. MDS42
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3976195)
  AUTHORS   Ying,B.W., Seno,S., Suzuki,S. and Yomo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Shigeto Seno
            Osaka University, Graduate School of Information Science and
            Technology; Yamadaoka 1-5, Suita, Osaka 565-0871, Japan
REFERENCE   2  
  AUTHORS   Fredens,J., Wang,K., de la Torre,D., Funke,L.F.H., Robertson,W.E.,
            Christova,Y., Chia,T., Schmied,W.H., Dunkelmann,D.L., Beranek,V.,
            Uttamapinant,C., Llamazares,A.G., Elliott,T.S. and Chin,J.W.
  TITLE     Total synthesis of Escherichia coli with a recoded genome
  JOURNAL   Nature 569, 514-518 (2019)
  REMARK    DOI:10.1038/s41586-019-1192-5
COMMENT     MDS42 is a genome reduced strain constructed from MG1655 by Posfai
            et al (originally reported in Science 2006), and is commercially
            available from Scarab Genomics (the founder is Dr. Frederick R.
            Blattner). We re-confirmed the whole genome sequence of MDS42 by
            the next generation resequencing technology based on SOLiD3
            (Applied Biosystems)
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: Bioscope v. 1.3
            Genome Coverage       :: 300x
            Sequencing Technology :: SOLiD 3
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /db_xref="taxon:1110693"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli str. K-12 substr. MDS42"
                     /strain="K-12"
                     /sub_strain="MDS42"
     protein         /gene="mdtA"
                     /gene_synonym="ECK2070"
                     /gene_synonym="JW5338"
                     /gene_synonym="b2074"
                     /locus_tag="ECMDS42_1657"
                     /transl_table=11
BEGIN
        1 MKGSYKSRWV IVIVVVIAAI AAFWFWQGRN DSRSAAPGAT KQAQQSPAGG RRGMRSGPLA
       61 PVQAATAVEQ AVPRYLTGLG TITAANTVTV RSRVDGQLIA LHFQEGQQVK AGDLLAEIDP
      121 SQFKVALAQA QGQLAKDKAT LANARRDLAR YQQLAKTNLV SRQELDAQQA LVSETEGTIK
      181 ADEASVASAQ LQLDWSRITA PVDGRVGLKQ VDVGNQISSG DTTGIVVITQ THPIDLVFTL
      241 PESDIATVVQ AQKAGKPLVV EAWDRTNSKK LSEGTLLSLD NQIDATTGTI KVKARFNNQD
      301 DALFPNQFVN ARMLVDTEQN AVVIPTAALQ MGNEGHFVWV LNSENKVSKH LVTPGIQDSQ
      361 KVVIRAGISA GDRVVTDGID RLTEGAKVEV VEAQSATTPE EKATSREYAK KGARS
//