LOCUS       BAL37537.1               414 aa    PRT              BCT 04-JUN-2019
DEFINITION  Escherichia coli str. K-12 substr. MDS42 hydrolase, binds
            to enzyme IIA(Glc) protein.
ACCESSION   AP012306-217
PROTEIN_ID  BAL37537.1
SOURCE      Escherichia coli str. K-12 substr. MDS42
  ORGANISM  Escherichia coli str. K-12 substr. MDS42
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3976195)
  AUTHORS   Ying,B.W., Seno,S., Suzuki,S. and Yomo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Shigeto Seno
            Osaka University, Graduate School of Information Science and
            Technology; Yamadaoka 1-5, Suita, Osaka 565-0871, Japan
REFERENCE   2  
  AUTHORS   Fredens,J., Wang,K., de la Torre,D., Funke,L.F.H., Robertson,W.E.,
            Christova,Y., Chia,T., Schmied,W.H., Dunkelmann,D.L., Beranek,V.,
            Uttamapinant,C., Llamazares,A.G., Elliott,T.S. and Chin,J.W.
  TITLE     Total synthesis of Escherichia coli with a recoded genome
  JOURNAL   Nature 569, 514-518 (2019)
  REMARK    DOI:10.1038/s41586-019-1192-5
COMMENT     MDS42 is a genome reduced strain constructed from MG1655 by Posfai
            et al (originally reported in Science 2006), and is commercially
            available from Scarab Genomics (the founder is Dr. Frederick R.
            Blattner). We re-confirmed the whole genome sequence of MDS42 by
            the next generation resequencing technology based on SOLiD3
            (Applied Biosystems)
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: Bioscope v. 1.3
            Genome Coverage       :: 300x
            Sequencing Technology :: SOLiD 3
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /db_xref="taxon:1110693"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli str. K-12 substr. MDS42"
                     /strain="K-12"
                     /sub_strain="MDS42"
     protein         /gene="frsA"
                     /gene_synonym="ECK0240"
                     /gene_synonym="JW0229"
                     /gene_synonym="b0239"
                     /locus_tag="ECMDS42_0228"
                     /transl_table=11
BEGIN
        1 MTQANLSETL FKPRFKHPET STLVRRFNHG AQPPVQSALD GKTIPHWYRM INRLMWIWRG
       61 IDPREILDVQ ARIVMSDAER TDDDLYDTVI GYRGGNWIYE WATQAMVWQQ KACAEDDPQL
      121 SGRHWLHAAT LYNIAAYPHL KGDDLAEQAQ ALSNRAYEEA AQRLPGTMRQ MEFTVPGGAP
      181 ITGFLHMPKG DGPFPTVLMC GGLDAMQTDY YSLYERYFAP RGIAMLTIDM PSVGFSSKWK
      241 LTQDSSLLHQ HVLKALPNVP WVDHTRVAAF GFRFGANVAV RLAYLESPRL KAVACLGPVV
      301 HTLLSDFKCQ QQVPEMYLDV LASRLGMHDA SDEALRVELN RYSLKVQGLL GRRCPTPMLS
      361 GYWKNDPFSP EEDSRLITSS SADGKLLEIP FNPVYRNFDK GLQEITDWIE KRLC
//