LOCUS BAE01613.1 434 aa PRT PRI 06-OCT-2006 DEFINITION Macaca fascicularis protein ( Macaca fascicularis brain cDNA, clone: QccE-14518, similar to human enolase 1, ( alpha) (ENO1), mRNA, RefSeq: NM_001428.2. ). ACCESSION AB169531-1 PROTEIN_ID BAE01613.1 SOURCE Macaca fascicularis (crab-eating macaque) ORGANISM Macaca fascicularis Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Macaca. REFERENCE 1 (bases 1 to 1852) AUTHORS Hashimoto,K., Kusuda,J. and Sugano,S. TITLE Direct Submission JOURNAL Submitted (18-MAR-2004) to the DDBJ/EMBL/GenBank databases. Contact:Katsuyuki Hashimoto National Institute of Infectious Diseases, Division of Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan URL :http://www.nih.go.jp/yoken/genebank/ REFERENCE 2 AUTHORS CONSRTM International consortium for macaque cDNA sequencing and analysis TITLE DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications JOURNAL Unpublished (2004) REFERENCE 3 AUTHORS Osada,N., Hirata,M., Tanuma,R., Kusuda,J., Hida,M., Suzuki,Y., Sugano,S., Gojobori,T., Shen,C.-K.J., Wu,C.I. and Hashimoto,K. TITLE Substitution Rate and Structural Divergence of 5'UTR Evolution: Comparative Analysis Between Human and Cynomolgus Monkey cDNAs JOURNAL Mol. Biol. Evol. 22, 1976-1982 (2005) COMMENT The International consortium for macaque cDNA sequencing and analysis consists of:Department of Virology and Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan; Division of Genetic Resources, National Institute of Infectious Diseases of Japan, Tokyo, Japan; National Health Research Institute, Taipei, Taiwan; Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & Evolution, University of Chicago, Chicago, IL, USA, Center for Information Biology, National Institute of Genetics of Japan, Mishima, Japan. Clone distribution: clone distribution information can be found at: http://www.nih.go.jp/yoken/genebank/ Lab host: TOP10 Vector: pME18S-FL3 (Acc.No. AB009864) R. Site1: DraIII (CACTGTGTG) R. Site2: DraIII (CACCATGTG) Description: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was synthesized using specific 5' and 3' primers and amplified by PCR. The PCR product was digested with SfiI and size selection was performed to exclude fragments <1.5kb.The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method. Libraries were made from: QccE: cerebellum cortex QnpA: parietal lobe QtrA: temporal lobe right QflA: frontal lobe left QmoA: medulla oblongata QbsA: brain stem QorA: occipital lobe right QtsA: testis Custom primers were used for 5' and 3'-end sequencing. The full-insert sequencing was done by primer-walking method using ABI DNA sequencer. FEATURES Qualifiers source /clone="QccE-14518" /clone_lib="macaque cDNA library QccE" /db_xref="taxon:9541" /dev_stage="adult" /mol_type="mRNA" /organism="Macaca fascicularis" /sex="male" protein /note="Homo sapiens enolase 1, (alpha) (ENO1), mRNA, RefSeq: NM_001428.2" BEGIN 1 MSILKVHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK 61 GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK 121 AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE 181 AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV 241 VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD 301 WGAWQKFTAS AGIQVVGDDL TVANPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA 361 QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK 421 AKFAGRNFRN PLAK //