LOCUS       AZN94426.1               687 aa    PRT              BCT 18-DEC-2018
DEFINITION  Pseudomonas aeruginosa bifunctional diguanylate cyclase/
            phosphodiesterase protein.
ACCESSION   CP032541-4590
PROTEIN_ID  AZN94426.1
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6264451)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the
            following genes: toxA, plcH, phzM, wapR, and aroA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6264451)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory,
            PO Box 1663 M888, Los Alamos, NM 87545, USA
COMMENT     Bacteria and source DNA available from Dr. Hongwei Yu.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date             :: 20-SEP-2018
            Assembly Method           :: Bowtie2 v. 2018-09-20
            Assembly Name             :: PGN5
            Genome Representation     :: Full
            Expected Final Version    :: Yes
            Reference-guided Assembly :: NC_002516.2
            Genome Coverage           :: 217.64x
            Sequencing Technology     :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/26/2018 16:28:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 5,903
            CDS (total)                       :: 5,840
            Genes (coding)                    :: 5,779
            CDS (coding)                      :: 5,779
            Genes (RNA)                       :: 63
            rRNAs                             :: 4 (5S)
            complete rRNAs                    :: 4 (5S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            Pseudo Genes (ambiguous residues) :: 12 of 61
            Pseudo Genes (frameshifted)       :: 22 of 61
            Pseudo Genes (incomplete)         :: 18 of 61
            Pseudo Genes (internal stop)      :: 13 of 61
            Pseudo Genes (multiple problems)  :: 4 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="PGN5"
                     /db_xref="taxon:287"
                     /country="USA: WV, Huntington"
                     /collection_date="21-Nov-2017"
                     /collected_by="Progenesis Technologies, LLC"
                     /identified_by="Progenesis Technologies, LLC"
                     /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-"
     protein         /locus_tag="PGN5_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MKLDSRHSLS LKLLRVVLLA ALAVGVVLSC AQIVFDAYKA KQAVSSDAQR ILAMVRDPST
       61 QAVYSLDREM AMQVLEGLFQ HEAVRQASIG HPGEPMLAEK SRPLLDLPTR WLTDPILGQE
      121 RTFSIRLIGR PPYSEYYGDL KITLDTAPYG ENFVTTSEII FISGILRALA MGLVLFLVYH
      181 WMLTKPLSKI IEHLVSINPD RPSQHQLPLL KGHERNELGL WVTTANQLLA SIESNSHLRR
      241 EAEDNLLRIS QYDFLTGLPN RQLLQQQLDQ ILDGAGRQQR RVAVLCLGLD DFKGINEQYT
      301 YQLGDQLLIA LADRLRGHSA RLGSLARLGG DQFALVQADI EQPYEAAELA QSILDGLEAP
      361 FEIDQHEVRL RATIGITLFP EDGETTEKLL QKAEQTMTLA KTRSRNRYQF YIASVDSEMR
      421 RRRELEKDLR DALQRHELHL VYQPQVDYRD HRVVGVEALL RWQHPLHGFV PPDLFIPLAE
      481 QNGSIFSIGE WVLDQACRQL REWHDQGFDD LRMAVNLSTV QLHHNALPRV VSNLLQVYRL
      541 PARSLELEVT ETGLMEDIST AAQHLLSLRR AGALIAIDDF GTGYSSLSYL KSLPLDKIKI
      601 DKSFVQDLLQ DEDDATIVRA IIQLGKSLGM QVIAEGVETA EQEAYIIAEG CNEGQGYLYS
      661 KPLPARELTQ YLKQARRLSQ ATSSERP
//