LOCUS       AZN92431.1               668 aa    PRT              BCT 18-DEC-2018
DEFINITION  Pseudomonas aeruginosa type VI secretion system tip protein
            VgrG protein.
ACCESSION   CP032541-2497
PROTEIN_ID  AZN92431.1
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6264451)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the
            following genes: toxA, plcH, phzM, wapR, and aroA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6264451)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory,
            PO Box 1663 M888, Los Alamos, NM 87545, USA
COMMENT     Bacteria and source DNA available from Dr. Hongwei Yu.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date             :: 20-SEP-2018
            Assembly Method           :: Bowtie2 v. 2018-09-20
            Assembly Name             :: PGN5
            Genome Representation     :: Full
            Expected Final Version    :: Yes
            Reference-guided Assembly :: NC_002516.2
            Genome Coverage           :: 217.64x
            Sequencing Technology     :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/26/2018 16:28:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 5,903
            CDS (total)                       :: 5,840
            Genes (coding)                    :: 5,779
            CDS (coding)                      :: 5,779
            Genes (RNA)                       :: 63
            rRNAs                             :: 4 (5S)
            complete rRNAs                    :: 4 (5S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            Pseudo Genes (ambiguous residues) :: 12 of 61
            Pseudo Genes (frameshifted)       :: 22 of 61
            Pseudo Genes (incomplete)         :: 18 of 61
            Pseudo Genes (internal stop)      :: 13 of 61
            Pseudo Genes (multiple problems)  :: 4 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="PGN5"
                     /db_xref="taxon:287"
                     /country="USA: WV, Huntington"
                     /collection_date="21-Nov-2017"
                     /collected_by="Progenesis Technologies, LLC"
                     /identified_by="Progenesis Technologies, LLC"
                     /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-"
     protein         /gene="tssI"
                     /locus_tag="PGN5_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MPRPTDTNTT LSLSASTLGD LYPQTLSGGE ALNELGSLTL GGYSATALTL SNAVATHLTA
       61 TLHNDADQRP LDGLVAEIRQ LPGDASAERY QVLLRPWLWW LTLASNNRVF QNLATSDIVT
      121 QVFDQHGFSD YQLQLSGSYQ PREYCVQYGE SDLAFVSRLL EEDGIFWFFT HAAGKHTLVL
      181 ADSNDAFPPI PNGPQVAYLG QGIGVRELQG VRSAQYSLQA VSGTYSATDY EFTTPGTSLY
      241 SQAEAVSGAA GVYQHPGGYT AKAQGDSLTK QRIDGLRSQE TRLIGESDCR WLVPGHWFTL
      301 SGHDDDSLNI DWVLTSVTHD ASHAHYRNRF EAIPKATAYR PARVTPKPRM HTQTALVVGK
      361 AGEEIWTDQY GRIKIQFPWD RDGKNDETSS CWVRVVLPWS GKGFGMQFVP RIGQEVIVTF
      421 IDGDPDRPLV TGCVYNGDNA LPYALPDNQT QSGIKTNSSK GGGGFNELRF EDKKDAEEVF
      481 LQAQKDLNVN VLNDSTASIG HDETLTVQNA RTRTVKEGDE TVTLEKGKRT VTIQTGSDSL
      541 DVKDTRTVTV GADQTHSTGG NYSHKVSGNF ELTVDGNLTI KVSGTLALQS GGSLTLKSDA
      601 DLAAQAGTSL TSKAGTSLTN QAGTSLTNKA GTSLTNDAGV SLTNKAGAEQ TVDGGGMLTI
      661 KGGLVKVN
//