LOCUS       AZN90472.1              1046 aa    PRT              BCT 18-DEC-2018
DEFINITION  Pseudomonas aeruginosa multidrug efflux RND transporter
            permease subunit MexB protein.
ACCESSION   CP032541-450
PROTEIN_ID  AZN90472.1
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6264451)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the
            following genes: toxA, plcH, phzM, wapR, and aroA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6264451)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory,
            PO Box 1663 M888, Los Alamos, NM 87545, USA
COMMENT     Bacteria and source DNA available from Dr. Hongwei Yu.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date             :: 20-SEP-2018
            Assembly Method           :: Bowtie2 v. 2018-09-20
            Assembly Name             :: PGN5
            Genome Representation     :: Full
            Expected Final Version    :: Yes
            Reference-guided Assembly :: NC_002516.2
            Genome Coverage           :: 217.64x
            Sequencing Technology     :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/26/2018 16:28:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 5,903
            CDS (total)                       :: 5,840
            Genes (coding)                    :: 5,779
            CDS (coding)                      :: 5,779
            Genes (RNA)                       :: 63
            rRNAs                             :: 4 (5S)
            complete rRNAs                    :: 4 (5S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            Pseudo Genes (ambiguous residues) :: 12 of 61
            Pseudo Genes (frameshifted)       :: 22 of 61
            Pseudo Genes (incomplete)         :: 18 of 61
            Pseudo Genes (internal stop)      :: 13 of 61
            Pseudo Genes (multiple problems)  :: 4 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="PGN5"
                     /db_xref="taxon:287"
                     /country="USA: WV, Huntington"
                     /collection_date="21-Nov-2017"
                     /collected_by="Progenesis Technologies, LLC"
                     /identified_by="Progenesis Technologies, LLC"
                     /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-"
     protein         /locus_tag="PGN5_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MSKFFIDRPI FAWVIALVIM LAGGLSILSL PVNQYPAIAP PAIAVQVSYP GASAETVQDT
       61 VVQVIEQQMN GIDNLRYISS ESNSDGSMTI TVTFEQGTDP DIAQVQVQNK LQLATPLLPQ
      121 EVQRQGIRVT KAVKNFLMVV GVVSTDGSMT KEDLSNYIVS NIQDPLSRTK GVGDFQVFGS
      181 QYSMRIWLDP AKLNSYQLTP GDVSSAIQAQ NVQISSGQLG GLPAVKGQQL NATIIGKTRL
      241 QTAEQFENIL LKVNPDGSQV RLKDVADVGL GGQDYSINAQ FNGSPASGIA IKLATGANAL
      301 DTAKAIRQTI ANLEPFMPQG MKVVYPYDTT PVVSASIHEV VKTLGEAILL VFLVMYLFLQ
      361 NFRATLIPTI AVPVVLLGTF GVLAAFGFSI NTLTMFGMVL AIGLLVDDAI VVVENVERVM
      421 AEEGLSPREA ARKSMGQIQG ALVGIAMVLS AVFLPMAFFG GSTGVIYRQF SITIVSAMAL
      481 SVIVALILTP ALCATMLKPI EKGDHGEHKG GFFGWFNRMF LSTTHGYERG VASILKHRAP
      541 YLLIYVVIVA GMIWMFTRIP TAFLPDEDQG VLFAQVQTPP GSSAERTQVV VDSMREYLLE
      601 KESSSVSSVF TVTGFNFAGR GQSSGMAFIM LKPWEERPGG ENSVFELAKR AQMHFFSFKD
      661 AMVFAFAPPS VLELGNATGF DLFLQDQAGV GHEVLLQARN KFLMLAAQNP ALQRVRPNGM
      721 SDEPQYKLEI DDEKASALGV SLADINSTVS IAWGSSYVND FIDRGRVKRV YLQGRPDARM
      781 NPDDLSKWYV RNDKGEMVPF NAFATGKWEY GSPKLERYNG VPAMEILGEP APGLSSGDAM
      841 AAVEEIVKQL PKGVGYSWTG LSYEERLSGS QAPALYALSL LVVFLCLAAL YESWSIPFSV
      901 MLVVPLGVIG ALLATSMRGL SNDVFFQVGL LTTIGLSAKN AILIVEFAKE LHEQGKGIVE
      961 AAIEACRMRL RPIVMTSLAF ILGVVPLAIS TGAGSGSQHA IGTGVIGGMV TATVLAIFWV
     1021 PLFYVAVSTL FKDEASKQQA SVEKGQ
//