LOCUS       AZN90342.1               367 aa    PRT              BCT 18-DEC-2018
DEFINITION  Pseudomonas aeruginosa putrescine-binding periplasmic
            protein SpuD protein.
ACCESSION   CP032541-317
PROTEIN_ID  AZN90342.1
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6264451)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the
            following genes: toxA, plcH, phzM, wapR, and aroA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6264451)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory,
            PO Box 1663 M888, Los Alamos, NM 87545, USA
COMMENT     Bacteria and source DNA available from Dr. Hongwei Yu.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date             :: 20-SEP-2018
            Assembly Method           :: Bowtie2 v. 2018-09-20
            Assembly Name             :: PGN5
            Genome Representation     :: Full
            Expected Final Version    :: Yes
            Reference-guided Assembly :: NC_002516.2
            Genome Coverage           :: 217.64x
            Sequencing Technology     :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/26/2018 16:28:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 5,903
            CDS (total)                       :: 5,840
            Genes (coding)                    :: 5,779
            CDS (coding)                      :: 5,779
            Genes (RNA)                       :: 63
            rRNAs                             :: 4 (5S)
            complete rRNAs                    :: 4 (5S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            Pseudo Genes (ambiguous residues) :: 12 of 61
            Pseudo Genes (frameshifted)       :: 22 of 61
            Pseudo Genes (incomplete)         :: 18 of 61
            Pseudo Genes (internal stop)      :: 13 of 61
            Pseudo Genes (multiple problems)  :: 4 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="PGN5"
                     /db_xref="taxon:287"
                     /country="USA: WV, Huntington"
                     /collection_date="21-Nov-2017"
                     /collected_by="Progenesis Technologies, LLC"
                     /identified_by="Progenesis Technologies, LLC"
                     /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-"
     protein         /locus_tag="PGN5_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MMKRFGKTLL ALTLAGSVAG MAQAADNKVL HVYNWSDYIA PDTLEKFTKE TGIKVVYDVY
       61 DSNEVLEAKL LAGKSGYDVV VPSNSFLAKQ IKAGVYQKLD KSKLPNWKNL NKDLMHTLEV
      121 SDPGNEHAIP YMWGTIGIGY NPDKVKAAFG DNAPVDSWDL VFKPENIQKL KQCGVSFLDS
      181 PTEILPAALH YLGYKPDTDN PKELKAAEEL FLKIRPYVTY FHSSKYISDL ANGNICVAIG
      241 YSGDIYQAKS RAEEAKNKVT VKYNIPKEGA GSFFDMVAIP KDAENTEGAL AFVNFLMKPE
      301 IMAEITDVVQ FPNGNAAATP LVSEAIRNDP GIYPSEEVMK KLYTFPDLPA KTQRAMTRSW
      361 TKIKSGK
//