LOCUS       AZN90176.1               497 aa    PRT              BCT 18-DEC-2018
DEFINITION  Pseudomonas aeruginosa methylmalonate-semialdehyde dehydrogenase
            (CoA acylating) protein.
ACCESSION   CP032541-141
PROTEIN_ID  AZN90176.1
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6264451)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the
            following genes: toxA, plcH, phzM, wapR, and aroA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6264451)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory,
            PO Box 1663 M888, Los Alamos, NM 87545, USA
COMMENT     Bacteria and source DNA available from Dr. Hongwei Yu.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date             :: 20-SEP-2018
            Assembly Method           :: Bowtie2 v. 2018-09-20
            Assembly Name             :: PGN5
            Genome Representation     :: Full
            Expected Final Version    :: Yes
            Reference-guided Assembly :: NC_002516.2
            Genome Coverage           :: 217.64x
            Sequencing Technology     :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/26/2018 16:28:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 5,903
            CDS (total)                       :: 5,840
            Genes (coding)                    :: 5,779
            CDS (coding)                      :: 5,779
            Genes (RNA)                       :: 63
            rRNAs                             :: 4 (5S)
            complete rRNAs                    :: 4 (5S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            Pseudo Genes (ambiguous residues) :: 12 of 61
            Pseudo Genes (frameshifted)       :: 22 of 61
            Pseudo Genes (incomplete)         :: 18 of 61
            Pseudo Genes (internal stop)      :: 13 of 61
            Pseudo Genes (multiple problems)  :: 4 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="PGN5"
                     /db_xref="taxon:287"
                     /country="USA: WV, Huntington"
                     /collection_date="21-Nov-2017"
                     /collected_by="Progenesis Technologies, LLC"
                     /identified_by="Progenesis Technologies, LLC"
                     /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-"
     protein         /gene="mmsA"
                     /locus_tag="PGN5_00705"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MGTLHHLING EMVADNGRSA DVFNPSTGEA IHKVPLADGK TLQKAIDAAR AAFPAWRNTP
       61 PAKRAQVLYR FKQLLEQNEA RISKLISEEH GKTLEDAAGE LKRGIENVEY ACAAPEILKG
      121 EYSRNVGPNI DAWSDFQPIG VVAGITPFNF PAMVPLWMYP LAIACGNTFI LKPSERDPSS
      181 TLLIAELFHE AGLPKGVLNV VHGDKEAVDG LLQAPEVKAI SFVGSTPIAE YIYAEGTKRG
      241 KRVQALGGAK NHAVLMPDAD LDNAVSALMG AAYGSCGERC MAISVAVCVG DQVADALIAK
      301 LVPQIKALKI GAGTSCGLDM GPLVTAAAQA KVTGYIDSGV AQGAELVVDG RGYQVAGHEN
      361 GFFLGGSLFD RVTPEMTIYK EEIFGPVLCV VRVNSLEEAM QLINDHEYGN GTCIFTRDGE
      421 AARLFCDEIE VGMVGVNVPL PVPVAYHSFG GWKRSLFGDL HAYGPDGVRF YTRRKAITQR
      481 WPQRASHEAS QFAFPSL
//