LOCUS       AZN84751.1               310 aa    PRT              BCT 18-DEC-2018
DEFINITION  Pseudomonas aeruginosa malonate decarboxylase subunit
            epsilon protein.
ACCESSION   CP032540-228
PROTEIN_ID  AZN84751.1
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6264422)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the
            following genes: toxA, plcH, phzM, and wapR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6264422)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory,
            PO Box 1663 M888, Los Alamos, NM 87545, USA
COMMENT     Bacteria and source DNA available from Dr. Hongwei Yu
            (yuh@marshall.edu).
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date             :: 20-SEP-2018
            Assembly Method           :: Bowtie2 v. 2018-09-20
            Genome Representation     :: Full
            Expected Final Version    :: Yes
            Reference-guided Assembly :: NC_002516.2
            Genome Coverage           :: 368.17x
            Sequencing Technology     :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/26/2018 12:47:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 5,631
            CDS (total)                       :: 5,568
            Genes (coding)                    :: 5,507
            CDS (coding)                      :: 5,507
            Genes (RNA)                       :: 63
            rRNAs                             :: 4 (5S)
            complete rRNAs                    :: 4 (5S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            Pseudo Genes (ambiguous residues) :: 13 of 61
            Pseudo Genes (frameshifted)       :: 21 of 61
            Pseudo Genes (incomplete)         :: 18 of 61
            Pseudo Genes (internal stop)      :: 13 of 61
            Pseudo Genes (multiple problems)  :: 4 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="PGN4"
                     /isolation_source="derived from laboratory strain"
                     /db_xref="taxon:287"
                     /country="USA: WV, Huntington"
                     /collection_date="13-Sep-2014"
                     /collected_by="Progenesis Technologies, LLC"
                     /identified_by="Progenesis Technologies, LLC"
                     /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-"
     protein         /gene="mdcH"
                     /locus_tag="PGN4_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MSVLFAYPGQ GAQRPGMLAA LPDEPPVRAC LEQAADCLGQ APAELESAEA LRGTRAVQLC
       61 LLIAGVAASR LLETRGHRPG LVAGLSIGAY PAAVVAGALD FDDALRLVAL RGELMQAAWP
      121 EGYGMSAILG LEQAQLEALI LAVRREHPPL YLANVNAERQ LVVAGSEAAL AALAERARAA
      181 GASAAKRLAV SVPSHCALLD EPAARLAEAF AGIRLHRPRV PYLSSSRARL VAEPAALADD
      241 LAGNMARRVE WLATLRSAYE RGARLHLELP PGRVLSGLAR PLFGCATPAF EGSRADTLDA
      301 LLREEEKRTR
//