LOCUS       ALR14598.1               316 aa    PRT              BCT 02-SEP-2016
DEFINITION  Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 glutathione
            synthetase protein.
ACCESSION   CP009977-127
PROTEIN_ID  ALR14598.1
SOURCE      Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759
  ORGANISM  Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 3248023)
  AUTHORS   Lee,H. and Church,G.M.
  TITLE     Vibrio natriegens genome v1.0
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3248023)
  AUTHORS   Lee,H. and Church,G.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2014) Genetics, Harvard Medical School, 77 Avenue
            Louis Pasteur, NRB 233, Boston, MA 02115, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: RS HGAP v. 3
            Assembly Name          :: Vnat1.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 96.48x
            Sequencing Technology  :: PacBio
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 11/24/2014 10:39:19
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS+
            Annotation Software revision :: 2.9 (rev. 452286)
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA;
                                            repeat_region
            Genes                        :: 4,665
            CDS                          :: 4,459
            Pseudo Genes                 :: 42
            rRNAs                        :: 34 (5S, 16S, 23S)
            tRNAs                        :: 129
            ncRNA                        :: 1
            Frameshifted Genes           :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM
                     759"
                     /mol_type="genomic DNA"
                     /strain="ATCC 14048"
                     /isolation_source="salt marsh mud"
                     /culture_collection="ATCC:14048"
                     /type_material="type strain of Vibrio natriegens"
                     /db_xref="taxon:1219067"
                     /chromosome="1"
                     /country="USA"
                     /collection_date="27-Feb-2012"
     protein         /locus_tag="PN96_00855"
                     /EC_number="6.3.2.3"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:Q87LK1.1"
                     /note="catalyzes the second step in the glutathione
                     biosynthesis pathway, where it synthesizes ATP +
                     gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate +
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MIKLGIVMDP ISSINIKKDS SFAMMLEAQR RGYEIHYMEM NDLHLDQGVA IADTKVVELK
       61 EDPNGWYEFK SEQTIELSEL DAVLMRKDPP FDTEYIYATY ILERAEEQGT LIVNKPQSLR
      121 DCNEKLFTAW FPELTPTTIV TRKAEKIKAF REEHGDVILK PLDGMGGASI FRVNENDPNV
      181 SVIIETLTNH GQHYAMAQTF VPDISNGDKR ILVVDGEPMP YCLARIPAKG ETRGNLAAGG
      241 TGEARPLSET DIKIAQTVAP TLKEKGLIFV GLDVIGDKLT EINVTSPTCI REIEAAFDIS
      301 ITGKLMDAIE RRVKGE
//