LOCUS       AKO88932.1               158 aa    PRT              BCT 27-AUG-2015
DEFINITION  Pseudomonas aeruginosa DSM 50071 = NBRC 12689 transcription
            elongation factor GreA protein.
ACCESSION   CP012001-4877
PROTEIN_ID  AKO88932.1
SOURCE      Pseudomonas aeruginosa DSM 50071 = NBRC 12689
  ORGANISM  Pseudomonas aeruginosa DSM 50071 = NBRC 12689
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6317050)
  AUTHORS   Nakano,K., Terabayashi,Y., Shiroma,A., Shimoji,M., Tamotsu,H.,
            Ashimine,N., Ohki,S., Shinzato,M., Teruya,K., Satou,K. and
            Hirano,T.
  TITLE     First Complete Genome Sequence of Pseudomonas aeruginosa (Schroeter
            1872) Migula 1900 (DSM 50071T), Determined Using PacBio
            Single-Molecule Real-Time Technology
  JOURNAL   Genome Announc 3 (4), e00932-15 (2015)
   PUBMED   26294631
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317050)
  AUTHORS   Nakano,K., Terabayashi,Y., Shiroma,A., Shimoji,M., Tamotsu,H.,
            Ashimine,N., Ohki,S., Shinzato,M., Teruya,K., Satou,K. and
            Hirano,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2015) Research and Development Division, Okinawa
            Institute of Advanced Sciences, Okinawa Biotechnology Business
            Support Center, Suite109, 5-1, Suzaki, Uruma, Okinawa 904-2234,
            Japan
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: HGAP3 v. 2.3.0
            Genome Coverage       :: 843x
            Sequencing Technology :: PacBio
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 07/02/2015 10:33:57
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS+
            Annotation Software revision :: 2.10
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA;
                                            repeat_region
            Genes                        :: 5,805
            CDS                          :: 5,710
            Pseudo Genes                 :: 18
            CRISPR Arrays                :: 3
            rRNAs                        :: 12 (5S, 16S, 23S)
            tRNAs                        :: 64
            ncRNA                        :: 1
            Frameshifted Genes           :: 11
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Pseudomonas aeruginosa DSM 50071 = NBRC 12689"
                     /mol_type="genomic DNA"
                     /strain="DSM 50071"
                     /culture_collection="DSM:50071"
                     /type_material="type strain of Pseudomonas aeruginosa"
                     /db_xref="taxon:1123015"
     protein         /gene="greA"
                     /locus_tag="PA50071_24790"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_009613057.1"
                     /note="necessary for efficient RNA polymerase
                     transcription elongation past template-encoded arresting
                     sites; arresting sites in DNA have the property of
                     trapping a certain fraction of elongating RNA polymerases
                     that pass through, resulting in locked ternary complexes.
                     Cleavage of the nascent transcript by cleavage factors
                     such as GreA or GreB allows the resumption of elongation
                     from the new 3'terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /transl_table=11
BEGIN
        1 MSKFPMTVQG ARALEEEVKH LKGVLRPQIS QAIAEARELG DLKENAEYHA AREQQGMVEA
       61 RIRDIEAKLS NAQVIDVTAI PHSGKVIFGT TVDIANVETD ETVTYQIVGD DEADIKGGKI
      121 SVNSPIARAL IGKTEGDAVL VRTPGGDVEY EIVEVRHI
//