LOCUS AFM04514.1 435 aa PRT BCT 31-AUG-2017 DEFINITION Bernardetia litoralis DSM 6794 ribosome-associated GTPase EngA protein. ACCESSION CP003345-2002 PROTEIN_ID AFM04514.1 SOURCE Bernardetia litoralis DSM 6794 ORGANISM Bernardetia litoralis DSM 6794 Bacteria; Bacteroidota; Cytophagia; Cytophagales; Bernardetiaceae; Bernardetia. REFERENCE 1 (bases 1 to 4919337) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Lu,M., Kyrpides,N., Mavromatis,K., Ivanova,N., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Lang,E., Kopitz,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Flexibacter litoralis DSM 6794 JOURNAL Unpublished REFERENCE 2 (bases 1 to 4919337) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Lu,M., Kyrpides,N., Mavromatis,K., Ivanova,N., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Lang,E., Kopitz,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (05-JUN-2012) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4089456 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Flexibacter litoralis DSM 6794 collection_date :: Missing lat_lon :: 32.840 -117.258 depth :: Missing alt_elev :: Missing country :: USA environment :: Marine num_replicons :: 1 ref_biomaterial :: DSM 6794, ATCC 23117, IFO 15988, NBRC 15988 biotic_relationship :: Free living trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi04020 Type Strain :: Yes Funding Program :: DOE-GEBA 2009 Gene Calling Method :: Prodigal, GenePRIMP Isolation Site :: Sea water aquarium outflow, La Jolla, California, U.S.A. Temperature Range :: Mesophile Gram Staining :: Gram- ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Bernardetia litoralis DSM 6794" /mol_type="genomic DNA" /strain="DSM 6794" /isolation_source="sea water aquarium outflow" /culture_collection="DSM:6794" /type_material="type strain of Bernardetia litoralis" /db_xref="taxon:880071" /country="USA: La Jolla, CA" /lat_lon="32.840 N 117.258 W" protein /locus_tag="Fleli_2132" /note="PFAM: GTPase of unknown function; TIGRFAM: ribosome-associated GTPase EngA; small GTP-binding protein domain" /transl_table=11 /db_xref="InterPro:IPR002917" /db_xref="InterPro:IPR015946" /db_xref="InterPro:IPR016484" BEGIN 1 MSNIVAIVGR PNVGKSTLFN RLVEERKAIM HDESGVTRDR HYGQSEWNGN FFTVIDTGGY 61 VVGSEDVFEK AIREQVEIAM EEASVILFMV DTHVGVNPLD EEFVKVLRRS RKPIYLVANK 121 VENFEHRTNA TEFYNLGIGE LYCVSSQTGT GTGDLLDDVV KNFEEIGDED PNAGLPKIAI 181 LGRPNVGKSS FLNILLGEER TIVSDISGTT RDSIDTHYKA YGQEFILTDT AGMRRKARVK 241 ENIEFYSVMR SLKALDDCDV CIVMIDATQK LESQDLTLIR LAADRYKGIV LIANKWDLVE 301 KDSKTAHEYT LEMQQSLGSA AWVPIIFTSM LTKQRVFQSI EKAIEVYEAR KQKISTSKLN 361 NILLPDIKRT PPPVHRGKNI NIKYITQLQG NAPLFAFFTN YPQHVHESYK RFLENKIREH 421 FGFEGVPIRI IFKEK //