LOCUS AET73005.1 519 aa PRT HTG 25-JUL-2016 DEFINITION Phaeocystis globosa virus 12T major capsid protein protein. ACCESSION HQ634147-158 PROTEIN_ID AET73005.1 SOURCE Phaeocystis globosa virus 12T ORGANISM Phaeocystis globosa virus 12T Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prymnesiovirus. REFERENCE 1 (bases 1 to 460002) AUTHORS Henn,M.R., Brussaard,C., Wommack,E., Levin,J., Malboeuf,C., Casali,M., Russ,C., Lennon,N., Chapman,S.B., Erlich,R., Young,S.K., Yandava,C., Zeng,Q., Alvarado,L., Anderson,S., Berlin,A., Chen,Z., Freedman,E., Gellesch,M., Goldberg,J., Green,L., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D., Hollinger,A., Howarth,C., Larson,L., Mehta,T., Pearson,M., Roberts,A., Ryan,E., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., White,J., Haas,B., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform TITLE The Genome Sequence of Phaeocystis globosa virus 12T JOURNAL Unpublished REFERENCE 2 (bases 1 to 460002) AUTHORS Henn,M.R., Brussaard,C., Wommack,E., Levin,J., Malboeuf,C., Casali,M., Russ,C., Lennon,N., Chapman,S.B., Erlich,R., Young,S.K., Yandava,C., Zeng,Q., Alvarado,L., Anderson,S., Berlin,A., Chen,Z., Freedman,E., Gellesch,M., Goldberg,J., Green,L., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D., Hollinger,A., Howarth,C., Larson,L., Mehta,T., Pearson,M., Roberts,A., Ryan,E., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., White,J., Haas,B., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform TITLE Direct Submission JOURNAL Submitted (21-OCT-2010) Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA COMMENT This genome was sequenced by the Broad Institute and co-owned with CAMERA. ##Metadata-START## Isolation method :: double dilution series Template preparation method :: 1) Viral lysate filtered (2) concentrated virus particles embedded into PFGE agarose plug. (3) Viral genomic DNA PFGE-band B-agarase treated (4) DNA was ppt and resuspended in TE. 500ul from PFGE; 100ul covaris sheared MDA DNA; 40ul of MDA DNA Phage/virus type :: DNA:ds Viral DNA Phage/virus "hybrid" information :: not applicable Phage/virus taxonomy :: Phycodnaviridae Phage/virus strain :: 12 Morphology :: hexagonal, icosahedral symmetry Latitude :: 52.98N Longitude :: 4.74E Depth (m) :: 2.00 Collection date :: 05-Jul-2000 Sample collection site :: Atlantic Ocean: Southern North Sea Other collection site info :: not applicable Filter fraction (um) :: not applicable Volume filtered (L) :: not applicable Habitat description :: coastal water Other metadata available :: not applicable References :: not applicable Lab Host :: Phaeocystis globosa Pg-PI ##Metadata-END## * NOTE: This is a 'working draft' sequence. It currently * consists of 3 contigs. The true order of the pieces * is not known and their order in this sequence record is * arbitrary. Gaps between the contigs are represented as * runs of N, but the exact sizes of the gaps are unknown. * This record will be updated with the finished sequence * as soon as it is available and the accession number will * be preserved. * 1 6996: contig of 6996 bp in length * 6997 7096: gap of unknown length * 7097 39566: contig of 32470 bp in length * 39567 39666: gap of unknown length * 39667 460002: contig of 420336 bp in length. FEATURES Qualifiers source /organism="Phaeocystis globosa virus 12T" /mol_type="genomic DNA" /strain="12T" /db_xref="taxon:755273" /lab_host="Phaeocystis globosa Pg-PI" /country="Atlantic Ocean: Southern North Sea" /lat_lon="52.98 N 4.74 E" /collection_date="05-Jul-2000" protein /locus_tag="PGAG_00116" BEGIN 1 MAGGLMQLVA YGAQDVYLTG NPQITFWKVT YRRHTHFAME SIEQTFNGQA DFGRRVTCTI 61 SRNGDLAYRT YLQITLPEIG QSLSSGDVYA RWLDFPGEQL ISQVEVEIGG QRIDRQYGDW 121 MHIWNQLTLS KEQERGYYKM VGNTTQLTYV CDPTFAAVDG PCSANGVRQV CAPRNALPET 181 TLYVPLQFWY CRNPGLALPL IALQYHEVKI NLDIRNIEEC LWAVSAIDGT GTKITDAYKQ 241 SLAAASLFVD YIFLDTDERR RMAQNPHEYL IEQLQFTGDE SVGSSSNKIK LNLNHPCKEL 301 VWVVQPDANV DYCSSLTQNS HLNNLLGAQP FNYTDAFDAL PNAVHAFGGT QSVSGANGVI 361 NTSGYFEDPF ANDGAAVSAD PAGASADSGV SDAGTFVLAE TALDMHCWGE NPVIVAKLQL 421 NGQDRFSERE GTYFDLVQPF QHHTRAPDSG INVYSFALRP EEHQPSGSCN FSRIDNATLQ 481 LVLSNATVQG VNTAKVRVYA VNYNVLRIMS GMGGLAYSN //