LOCUS       AET72935.1               515 aa    PRT              HTG 25-JUL-2016
DEFINITION  Phaeocystis globosa virus 12T hypothetical protein protein.
ACCESSION   HQ634147-88
PROTEIN_ID  AET72935.1
SOURCE      Phaeocystis globosa virus 12T
  ORGANISM  Phaeocystis globosa virus 12T
            Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota;
            Megaviricetes; Algavirales; Phycodnaviridae; Prymnesiovirus.
REFERENCE   1  (bases 1 to 460002)
  AUTHORS   Henn,M.R., Brussaard,C., Wommack,E., Levin,J., Malboeuf,C.,
            Casali,M., Russ,C., Lennon,N., Chapman,S.B., Erlich,R., Young,S.K.,
            Yandava,C., Zeng,Q., Alvarado,L., Anderson,S., Berlin,A., Chen,Z.,
            Freedman,E., Gellesch,M., Goldberg,J., Green,L., Griggs,A.,
            Gujja,S., Heilman,E.R., Heiman,D., Hollinger,A., Howarth,C.,
            Larson,L., Mehta,T., Pearson,M., Roberts,A., Ryan,E., Saif,S.,
            Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., White,J.,
            Haas,B., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform
  TITLE     The Genome Sequence of Phaeocystis globosa virus 12T
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 460002)
  AUTHORS   Henn,M.R., Brussaard,C., Wommack,E., Levin,J., Malboeuf,C.,
            Casali,M., Russ,C., Lennon,N., Chapman,S.B., Erlich,R., Young,S.K.,
            Yandava,C., Zeng,Q., Alvarado,L., Anderson,S., Berlin,A., Chen,Z.,
            Freedman,E., Gellesch,M., Goldberg,J., Green,L., Griggs,A.,
            Gujja,S., Heilman,E.R., Heiman,D., Hollinger,A., Howarth,C.,
            Larson,L., Mehta,T., Pearson,M., Roberts,A., Ryan,E., Saif,S.,
            Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., White,J.,
            Haas,B., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2010) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     This genome was sequenced by the Broad Institute and co-owned with
            CAMERA.
            
            ##Metadata-START##
            Isolation method                 :: double dilution series
            Template preparation method      :: 1) Viral lysate filtered (2)
                                                concentrated virus particles
                                                embedded into PFGE agarose
                                                plug. (3) Viral genomic DNA
                                                PFGE-band B-agarase treated (4)
                                                DNA was ppt and resuspended in
                                                TE.
                                                500ul from PFGE; 100ul covaris
                                                sheared MDA DNA;
                                                40ul of MDA DNA
            Phage/virus type                 :: DNA:ds Viral DNA
            Phage/virus "hybrid" information :: not applicable
            Phage/virus taxonomy             :: Phycodnaviridae
            Phage/virus strain               :: 12
            Morphology                       :: hexagonal, icosahedral symmetry
            Latitude                         :: 52.98N
            Longitude                        :: 4.74E
            Depth (m)                        :: 2.00
            Collection date                  :: 05-Jul-2000
            Sample collection site           :: Atlantic Ocean: Southern North
                                                Sea
            Other collection site info       :: not applicable
            Filter fraction (um)             :: not applicable
            Volume filtered (L)              :: not applicable
            Habitat description              :: coastal water
            Other metadata available         :: not applicable
            References                       :: not applicable
            Lab Host                         :: Phaeocystis globosa Pg-PI
            ##Metadata-END##
            * NOTE: This is a 'working draft' sequence. It currently
            * consists of 3 contigs. The true order of the pieces
            * is not known and their order in this sequence record is
            * arbitrary. Gaps between the contigs are represented as
            * runs of N, but the exact sizes of the gaps are unknown.
            * This record will be updated with the finished sequence
            * as soon as it is available and the accession number will
            * be preserved.
            *        1     6996: contig of 6996 bp in length
            *     6997     7096: gap of unknown length
            *     7097    39566: contig of 32470 bp in length
            *    39567    39666: gap of unknown length
            *    39667   460002: contig of 420336 bp in length.
FEATURES             Qualifiers
     source          /organism="Phaeocystis globosa virus 12T"
                     /mol_type="genomic DNA"
                     /strain="12T"
                     /db_xref="taxon:755273"
                     /lab_host="Phaeocystis globosa Pg-PI"
                     /country="Atlantic Ocean: Southern North Sea"
                     /lat_lon="52.98 N 4.74 E"
                     /collection_date="05-Jul-2000"
     protein         /locus_tag="PGAG_00045"
BEGIN
        1 MSSVTKLYTE DIDKHKYSNG CVPKSAKKYA DPKTGAIDIE LWYEENPDSC QEFLNGAQKC
       61 SAEFYADDEK TGCCYNEKAK GDEWCKPDSW TGLNFMAADD TISNMCHKEA VQGMNNTLFD
      121 DNNKMIKFFK LALFSMISLL GAALVGTIYE FWLRYGNSVD CIYYKSKCAN IGKTDKISLI
      181 DYMFPNSLCH YPYQSCAKDK VTQSGGKKQS GGAKEVLTGI VSTFAEYERT GAKCITVNQD
      241 SGTVFGGKPV PYNIADYAIE NSDSEYMTVM AKTLSFYFLF SVLFTRKVLN AIMSKFSTGF
      301 QNNIKFNPFL SNLTFLLLTG IIFPILGYVL EMPGLYFGPM IIFTGLLILT SILTSIGFFV
      361 SFFSVIFPDK LWGSSMSECN LSAEYYRIFR PELLYTLKGA DMSAKVMSVV KNILQFLPLI
      421 LLILLSVVTG SLMCIVSSLY MSFSLFINMF LIPLSNPLEC FSILKSHADL LTVLFIMGVI
      481 GASANSLDQT TTGVMSMILV IIITLKAFKG MKNSI
//