LOCUS AET43521.1 945 aa PRT HTG 25-JUL-2016 DEFINITION Micromonas pusilla virus PL1 DNA polymerase protein. ACCESSION HQ633072-31 PROTEIN_ID AET43521.1 SOURCE Micromonas pusilla virus PL1 ORGANISM Micromonas pusilla virus PL1 Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prasinovirus. REFERENCE 1 (bases 1 to 197060) AUTHORS Henn,M.R., Suttle,C., Winget,D., Chan,A., Levin,J., Malboeuf,C., Casali,M., Russ,C., Lennon,N., Chapman,S.B., Erlich,R., Young,S.K., Yandava,C., Zeng,Q., Alvarado,L., Anderson,S., Berlin,A., Chen,Z., Freedman,E., Gellesch,M., Goldberg,J., Green,L., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D., Hollinger,A., Howarth,C., Larson,L., Mehta,T., Pearson,M., Roberts,A., Ryan,E., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., White,J., Haas,B., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform TITLE The Genome Sequence of Micromonas pusilla virus PL1 JOURNAL Unpublished REFERENCE 2 (bases 1 to 197060) AUTHORS Henn,M.R., Suttle,C., Winget,D., Chan,A., Levin,J., Malboeuf,C., Casali,M., Russ,C., Lennon,N., Chapman,S.B., Erlich,R., Young,S.K., Yandava,C., Zeng,Q., Alvarado,L., Anderson,S., Berlin,A., Chen,Z., Freedman,E., Gellesch,M., Goldberg,J., Green,L., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D., Hollinger,A., Howarth,C., Larson,L., Mehta,T., Pearson,M., Roberts,A., Ryan,E., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., White,J., Haas,B., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform TITLE Direct Submission JOURNAL Submitted (27-OCT-2010) Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA COMMENT This genome was sequenced by the Broad Institute and co-owned with CAMERA. ##Metadata-START## Isolation method :: double dilution series Template preparation method :: 0.45 micron filtered lysate followed by ultracentrifugation pelleting; Optiprep gradient; and Qiamp MinElute Virus DNA Spin Kit Phage/virus type :: DNA:ds Viral DNA Phage/virus "hybrid" information :: not applicable Phage/virus taxonomy :: Phycodnaviridae Phage/virus strain :: MpV-PL1 Morphology :: icosahedral; 113 nm diameter Latitude :: 27.83N Longitude :: 97.07W Depth (m) :: 1.00 Collection date :: 31-Oct-1990 Sample collection site :: Atlantic Ocean:Gulf of Mexico Other collection site info :: Sample collected off the University of Texas Marine Science Institute Pier in Aransas Pass, Texas, USA Filter fraction (um) :: 0.2 Volume filtered (L) :: 1.0 Habitat description :: coastal sea, mixed layer References :: PMID: 16535105; PMID: 16348439 Lab Host :: Micromonas pusilla ##Metadata-END## * NOTE: This is a 'working draft' sequence. It currently * consists of 2 contigs. The true order of the pieces * is not known and their order in this sequence record is * arbitrary. Gaps between the contigs are represented as * runs of N, but the exact sizes of the gaps are unknown. * This record will be updated with the finished sequence * as soon as it is available and the accession number will * be preserved. * 1 142634: contig of 142634 bp in length * 142635 142734: gap of unknown length * 142735 197060: contig of 54326 bp in length. FEATURES Qualifiers source /organism="Micromonas pusilla virus PL1" /mol_type="genomic DNA" /strain="PL1" /isolation_source="Atlantic Ocean: Gulf of Mexico" /db_xref="taxon:373997" /lab_host="Micromonas pusilla" /lat_lon="27.83 N 97.07 W" /collection_date="31-Oct-1990" protein /locus_tag="MPWG_00031" BEGIN 1 MVVFQALTWE ARDSDKEHLV SIFGKTEGGK SVCVTTSFTP YFFIKLDLKT SKQKIQEIYS 61 TIDRKCPECV LCYSMMKSKD VWGFQNNEEF MFMKVDFVNL QMRRRVDSFL KRPLELSSGF 121 FKAKVFESNL DPVLRLMHRT GIQSTGWLET GDNCIRSHLA RVDIDLFCND WTTLKPVARD 181 DIAPFVVASF DIECNSSTGK FPDADVTDDA CFQIAVSLCT FGSDEPYERV CLCYKETDGP 241 DTISFDTEKD MLEAFQKYIH EKDIDIFTGW NIFGFDLEFI YKRAFVVGCD PEFFKMGKLK 301 SQECELLIKK LSSSALGDNL LKLLPMSGRF IFDMFHEVKK GYKLDSYSLN NVSKLYIGDQ 361 KIDMPPREMF ARYREGDPVK LGEVAEYCIK DTLLPHKLMK KMCILLNLLE MAKATWVPMC 421 FLVERGQQIK VFSQLTKKAR ELGFMVPTIR YGTVTSDPYE GATVLEAQKG AYYTPITALD 481 FEALYPSIMM AHNLCYSSWV MNEKEYGNIP GVTYETFNVG EKTYKFAQGV PSLLPSILLE 541 LKQFRKKAKK DMATATGYMK EVYNGKQLAY KVSMNSVYGF TGAGKGILPC VPIASTTTFK 601 GRMMIEETKT YVEKNFPGAK VRYGDTDSVM VEFDVGDRKG VEAVEYSWEV GERAAEECSA 661 LFKKPNNLEL EKVYWPYFLY SKKRYAAKLW TKGKDDQMHM DYIDIKGLQV VRRDNTPHVR 721 EVCKELLDVV LTSSDPGPPT ELARERAIEL LSGDVPNEKL ILSQGLSDSY KVNGKSVSIN 781 SDESVGINQA HVQVVVKMRE RKPGSEPQSG DRVPYLLTNT GDRKAKAFEK SEDPKFVEEN 841 NIPVDYHYYF ENKFLNPVCD LLDPLFENTK QEIFGEIIDQ HKPPKKKREP ALSTMKKDQL 901 IEECKRLGLD ETGKLVDLRG RLKEARLKRE ESLEDIFKNY TQSNI //