LOCUS AEP16034.1 1251 aa PRT HTG 22-FEB-2012 DEFINITION Emiliania huxleyi virus 208 hypothetical protein protein. ACCESSION JF974318-156 PROTEIN_ID AEP16034.1 SOURCE Emiliania huxleyi virus 208 ORGANISM Emiliania huxleyi virus 208 Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Coccolithovirus. REFERENCE 1 (bases 1 to 411003) AUTHORS Nissimov,J.I., Worthy,C.A., Rooks,P., Napier,J.A., Kimmance,S.A., Henn,M.R., Ogata,H. and Allen,M.J. TITLE Draft Genome Sequence of Four Coccolithoviruses: Emiliania huxleyi Virus EhV-88, EhV-201, EhV-207, and EhV-208 JOURNAL J. Virol. 86 (5), 2896-2897 (2012) PUBMED 22328700 REFERENCE 2 (bases 1 to 411003) AUTHORS Henn,M.R., Allen,M., Levin,J., Malboeuf,C., Casali,M., Russ,C., Lennon,N., Chapman,S.B., Erlich,R., Young,S.K., Yandava,C., Zeng,Q., Alvarado,L., Anderson,S., Berlin,A., Chen,Z., Freedman,E., Gellesch,M., Goldberg,J., Green,L., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D., Hollinger,A., Howarth,C., Larson,L., Mehta,T., Pearson,M., Roberts,A., Ryan,E., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., White,J., Haas,B., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform TITLE The Genome Sequence of Emiliania huxleyi virus 208 JOURNAL Unpublished REFERENCE 3 (bases 1 to 411003) AUTHORS Henn,M.R., Allen,M., Levin,J., Malboeuf,C., Casali,M., Russ,C., Lennon,N., Chapman,S.B., Erlich,R., Young,S.K., Yandava,C., Zeng,Q., Alvarado,L., Anderson,S., Berlin,A., Chen,Z., Freedman,E., Gellesch,M., Goldberg,J., Green,L., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D., Hollinger,A., Howarth,C., Larson,L., Mehta,T., Pearson,M., Roberts,A., Ryan,E., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., White,J., Haas,B., Nusbaum,C. and Birren,B. CONSRTM The Broad Institute Genome Sequencing Platform TITLE Direct Submission JOURNAL Submitted (18-DEC-2010) Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA COMMENT This genome was sequenced by the Broad Institute and co-owned with CAMERA. ##Metadata-START## Isolation method :: triple-plaque-purified Template preparation method :: CsCl-gradient Phage/virus type :: DNA:ds Viral DNA Phage/virus "hybrid" information :: not applicable Phage/virus taxonomy :: Coccolithoviridae Phage/virus strain :: Strain 208 Morphology :: Icosahedral Latitude :: 50.25N Longitude :: 4.22W Depth (m) :: 5.00 Collection date :: 27-Jul-2007 Sample collection site :: English Channel Other collection site info :: L4 Filter fraction (um) :: 0.45 Volume filtered (L) :: 1.0 Habitat description :: Collected from natural E. huxleyi bloom Other metadata available :: http://www.westernchannelobserv atory.org.uk/data.php References :: PMID: 17359269; PMID: 16099989; PMID: 12209309; PMID: 16553948 Lab Host :: Emiliania huxleyi 1516 ##Metadata-END## * NOTE: This is a 'working draft' sequence. It currently * consists of 17 contigs. The true order of the pieces * is not known and their order in this sequence record is * arbitrary. Gaps between the contigs are represented as * runs of N, but the exact sizes of the gaps are unknown. * This record will be updated with the finished sequence * as soon as it is available and the accession number will * be preserved. * 1 85876: contig of 85876 bp in length * 85877 85976: gap of unknown length * 85977 149210: contig of 63234 bp in length * 149211 149310: gap of unknown length * 149311 201385: contig of 52075 bp in length * 201386 201485: gap of unknown length * 201486 242792: contig of 41307 bp in length * 242793 242892: gap of unknown length * 242893 277533: contig of 34641 bp in length * 277534 277633: gap of unknown length * 277634 302386: contig of 24753 bp in length * 302387 302486: gap of unknown length * 302487 324914: contig of 22428 bp in length * 324915 325014: gap of unknown length * 325015 345587: contig of 20573 bp in length * 345588 345687: gap of unknown length * 345688 363458: contig of 17771 bp in length * 363459 363558: gap of unknown length * 363559 374887: contig of 11329 bp in length * 374888 374987: gap of unknown length * 374988 383661: contig of 8674 bp in length * 383662 383761: gap of unknown length * 383762 389006: contig of 5245 bp in length * 389007 389106: gap of unknown length * 389107 393990: contig of 4884 bp in length * 393991 394090: gap of unknown length * 394091 398901: contig of 4811 bp in length * 398902 399001: gap of unknown length * 399002 403688: contig of 4687 bp in length * 403689 403788: gap of unknown length * 403789 407683: contig of 3895 bp in length * 407684 407783: gap of unknown length * 407784 411003: contig of 3220 bp in length. FEATURES Qualifiers source /organism="Emiliania huxleyi virus 208" /mol_type="genomic DNA" /strain="208" /isolation_source="English Channel" /db_xref="taxon:181215" /lab_host="Emiliania huxleyi 1516" /lat_lon="50.25 N 4.22 W" /collection_date="27-Jul-2007" protein /locus_tag="ERVG_00157" BEGIN 1 MAAKKNTSTK TKGKKDTQPA GKSNLRWYLM LTLKIIIALA VVLLFIYMYW RNQNEEARMF 61 AKIEQLKKEI GAQAASLSDS HAELSELNAE LEATELELKD SEEESVNLEK NIAEKTKEMM 121 ALQKQKELQR EQMRKANSEK MKEKQKEIDN LIAVYQSNID ALSANSPEEM KKIAAEKKIT 181 LDKLKESNAA EIAEMEQSYS KLATTVASDI ETRKNILSGE LEKELLKYDK QLETITAELE 241 PLAEKDGDEV KEKIKLLNDE IKVIGQKRAT AQSEHVAQLL SIETNKNNEL ADMQKTKRAS 301 INARKASQAS FIAKEDASYK AMLKNDENER NAMIDKHKKQ MEQLQFEKDS EIEDLEEQNN 361 SLDSDLSKLA DDLSVMKTQK EMLIIQKSDL QQAIEVHKKR DVEIKKLAGV YGCGSKTSTD 421 LANCTTKVTA NINAKKVERD NLISTITKKH ANGVASLKFH KGWKANSKYT PCPNGATLVG 481 GGPNDTYACV YSVGKFRSSQ STACVPGHGI PMTEKLVLPP GQMKQWFPRL PSCNYESTNV 541 WYIPWHFMRT YDEHIKMAKR YLVQLATFNT TEEWHMFMHF ERAYRTGRYF DQRGAYIVGT 601 EVVSEDGGFK GNPRSSESWK NYDDSVFFKD IPGNASAQPF KSVVILHEHP IGSRENNASI 661 GYGVGKYNLP GHLNDRVSSI LIHRGYSVHA FEHHDFKGHS EKFTGKVDLR GNRLERRISS 721 VIIEKVGDDV YDMIQKTKQK GIIGVAILEG KGIVPVPRNA RLPAIYKITA PTAALAAEAA 781 KLFMTKHGRQ RPDSIFALER GNDFDVYTVH KDRPLISENG RYELIIKDQR AQVGKVGGPY 841 TVISRNSKTD MIQIDVNGVL RFLDYAKTPN QTTMIGTKYL RLSNLGVLST NKHVLGNLSE 901 NNASGTLGTV NGSIAPTPEP KPPPKPPPKL PTQPRPMPSP KPPPPIAQLK LVEIVAFNSY 961 HQKEGYDQNF IGVPSYAVRD ISRAKVLCIG GVRHSVTHIM QTKDPKIPYV YLNPYLKRRV 1021 KVGTPVVIGD CNILTKPAPS SNKKGCHPNN CMVSTEHGAV AIQHLNVGDR IYTPSGYEPI 1081 IGFFDKDIKK TAEYYEITME NKQHITISKH HAIPINGRMT DPSLIKSGDV INTVSGITSV 1141 VSNNKVLQQG AHHFLVPSGL YYIDNVVCSD YTMHLPLVMF NLVHMYIRAR YNIGLPIIYR 1201 EQSVLSPYWP YHILGKLNAS TTMYNICSVL FVPLVILTEF ILAVYVSMNK K //