LOCUS ADU47016.1 811 aa PRT BCT 08-JAN-2015 DEFINITION Intrasporangium calvum DSM 43043 Peptidoglycan glycosyltransferase protein. ACCESSION CP002343-454 PROTEIN_ID ADU47016.1 SOURCE Intrasporangium calvum DSM 43043 ORGANISM Intrasporangium calvum DSM 43043 Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae; Intrasporangium. REFERENCE 1 (bases 1 to 4024382) AUTHORS Del Rio,T.G., Chertkov,O., Yasawong,M., Lucas,S., Deshpande,S., Cheng,J.F., Detter,C., Tapia,R., Han,C., Goodwin,L., Pitluck,S., Liolios,K., Ivanova,N., Mavromatis,K., Pati,A., Chen,A., Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D., Rohde,M., Pukall,R., Sikorski,J., Goker,M., Woyke,T., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Lapidus,A. TITLE Complete genome sequence of Intrasporangium calvum type strain (7 KIP) JOURNAL Stand Genomic Sci 3 (3), 294-303 (2010) PUBMED 21304734 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 4024382) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Tapia,R., Detter,J.C., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Intrasporangium calvum DSM 43043 JOURNAL Unpublished REFERENCE 3 (bases 1 to 4024382) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Tapia,R., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (17-NOV-2010) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4083316 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Intrasporangium calvum 7KIP, DSM 43043 collection_date :: 1967 lat_lon :: 55.753 37.604 depth :: Missing alt_elev :: Missing country :: Russia environment :: Air num_replicons :: Missing ref_biomaterial :: DSM 43043, ATCC 23552, CUB 430, IFO 12989, IMET 7816, JCM 3097, KCC A-0097, NBRC 12989, NCIB 10167 biotic_relationship :: Free living trophic_level :: Chemoorganotroph rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi02320 Type Strain :: Yes Greengenes ID :: 90541 Funding Program :: DOE-GEBA 2007 Number of Reads :: 847906 pyrosequencing, 11758818 Illumina Gene Calling Method :: Prodigal 1.4, GenePRIMP Isolation Site :: Air in a school dining room Isolation Comments :: isolated by L.V. Kalakoutskii Motility :: Nonmotile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 37C Gram Staining :: Gram+ Diseases :: None ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454-FLX, 454-Titanium, Illumina GAii ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Intrasporangium calvum DSM 43043" /mol_type="genomic DNA" /strain="DSM 43043" /isolation_source="air in a school dining room" /culture_collection="DSM:43043" /type_material="type strain of Intrasporangium calvum" /db_xref="taxon:710696" /country="Russia" /lat_lon="55.753 N 37.604 E" /collection_date="1967" /collected_by="L.V. Kalakoutskii" protein /locus_tag="Intca_0469" /EC_number="2.4.1.129" /note="COGs: COG0744 Membrane carboxypeptidase (penicillin-binding protein); InterPro IPR001264: IPR001460; KEGG: kse:Ksed_25450 membrane carboxypeptidase (penicillin-binding protein); PFAM: glycosyl transferase family 51; penicillin-binding protein transpeptidase; PRIAM: Peptidoglycan glycosyltransferase; SPTR: Putative transpeptidase; PFAM: Penicillin binding protein transpeptidase domain; Transglycosylase" /transl_table=11 BEGIN 1 MSSSRTTSIG GVISLLGAFL AAALVLGLLA AGLFMPAVGA TGAIARSGVD IFNELPGEFK 61 TDPLSQQSRI LAADGRVIAT PAEQNRIIVP LKNVAPIMRE AQVAIEDHRF YDHGGFDLQG 121 VVRAAIANLR TGQTTSGASS LTQQYVKISL QYSALNQGDK AAAQAAVRKD YMRKLKELKY 181 AITLEKKMTK DEILQGYLNL VYYGDQAYGV EAASRHYFSI PASKLSLSQA ALLAGLTQNP 241 GTTDPVNYPE RALARRNVVL DRMHELGRIS DKEWTDAKKR TLKQDLKVSQ PKSTCLASPY 301 PYWCEFVINY VETMPELGKT VEARRRLLRT GGITVQTTLD PKIQRIVQEE VTRKVPIGDP 361 SGVGAAAYVT EPGTGRVLAF AQNTKYTVGK QSRGKTGVSW ALDKRWGGSG GFQFGSTAKA 421 FSLLTAMESG MSTKASVKAK AAGDKPATYQ PSEFAKGCGP GTEWKVLNDT PFKGGSISLM 481 KATALSTNTA FVALAQQLGG CKVWDTMNRL GLRTASGQPV PKYAPAYILG AAEVSPQGVA 541 HAYATLAADG KKCSMVVVTK VTQGSKTLAA PKTKCDQVAK ADYVRGTDKF LEYNMTNGSG 601 VRNQLDGRES AGKTGTSDKN NESWFVGYTP NLVTSVWVGT PDDGNKRLMK NVRVGGQFYP 661 VMHGASIAAP IWKGIMNRAL EGEPVLRFKE PSDKVLNGEL VDIPSVTGRT VADATKILEE 721 AGFHRRRRRL DGVEHPQGPR RRHVTVRPGT PGQRRHDLHV RGPEPADEQG QARSEADHHD 781 RAAPRPEADE ATAPQAQLHG DRGPAQRLRA G //