LOCUS ACV56057.1 475 aa PRT BCT 11-DEC-2013 DEFINITION Eggerthella lenta DSM 2243 UDP-N-acetylmuramate/alanine ligase protein. ACCESSION CP001726-2062 PROTEIN_ID ACV56057.1 SOURCE Eggerthella lenta DSM 2243 ORGANISM Eggerthella lenta DSM 2243 Bacteria; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Eggerthella. REFERENCE 1 (bases 1 to 3632260) AUTHORS Saunders,E., Pukall,R., Abt,B., Lapidus,A., Glavina Del Rio,T., Copeland,A., Tice,H., Cheng,J.F., Lucas,S., Chen,F., Nolan,M., Bruce,D., Goodwin,L., Pitluck,S., Ivanova,N., Mavromatis,K., Ovchinnikova,G., Pati,A., Chen,A., Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D., Chain,P., Meincke,L., Sims,D., Brettin,T., Detter,J.C., Goker,M., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Han,C. TITLE Complete genome sequence of Eggerthella lenta type strain (IPP VPI 0255) JOURNAL Stand Genomic Sci 1 (2), 174-182 (2009) PUBMED 21304654 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 3632260) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Ovchinnikova,G., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Pukall,R., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Eggerthella lenta DSM 2243 JOURNAL Unpublished REFERENCE 3 (bases 1 to 3632260) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Ovchinnikova,G., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Pukall,R., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (02-SEP-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4082980 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Eggerthella lenta VPI 0255, DSM 2243 Culture Collection ID :: DSM 2243, ATCC 25559, JCM 9979 GOLD Stamp ID :: Gc01054 Funding Program :: DOE-GEBA 2007 Number of Reads :: 39464-Sanger, 471609-454 Assembly Method :: Newbler version 1.1.02.15, Phrap Sequencing Depth :: 10.2x Sanger; 25.3x 454 Gene Calling Method :: Prodigal Isolation Site :: Rectal tumor Collection Date :: 1938 Host Name :: Homo sapiens Host Health :: Patient with rectal tumor Body Sample Site :: Blood Oxygen Requirement :: Anaerobe Cell Shape :: Rod-shaped Motility :: Nonmotile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 37C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: Bacteremia Habitat :: Blood, Host, Human intestinal microflora Phenotypes :: Pathogen ##Metadata-END## FEATURES Qualifiers source /organism="Eggerthella lenta DSM 2243" /mol_type="genomic DNA" /strain="DSM 2243" /culture_collection="DSM:2243" /type_material="type strain of Eggerthella lenta" /db_xref="taxon:479437" protein /locus_tag="Elen_2094" /inference="protein motif:TFAM:TIGR01082" /note="TIGRFAM: UDP-N-acetylmuramate/alanine ligase; PFAM: cytoplasmic peptidoglycan synthetase domain protein; Mur ligase middle domain protein; KEGG: nwi:Nwi_1052 UDP-N-acetylmuramate--L-alanine ligase" /transl_table=11 /db_xref="InterPro:IPR000713" /db_xref="InterPro:IPR004101" /db_xref="InterPro:IPR005758" /db_xref="InterPro:IPR013221" BEGIN 1 MDTMHIDQVH FIGVGGVGMS GIARVAFDQG MRVSGTDLKE SRYTKQLREA GIEVHIGHDP 61 ANIPEGNPVI VVSTAILDNN PELAAAKARG LTIWHRAQML AYLGRALQTL AVAGTHGKTT 121 TSSMLASVLD GMGEDPTFLI GGIVRAYGTN AHSGTGAHYV VEADESDKSF MHLSPRAVLV 181 TNIEADHLDH YRDLDEIYEK FASFIGSVPD DGGVVVACGE DEALVRVARA SGKRLFTYGF 241 SDACDVRIAS YEPNGVGSDF TLELPDGSVV SAHVKQNPGR HNVLNAAGVV GLLWALGYDP 301 AQAAEALADF AGVKRRFDLV GEAGGVTVVD DYAHHPTEIA ATIEAASHLG FAHVHVLFQP 361 HRYSRAPLFT EVLKDDFGRA FDAADAVTFM DVYPAGEAPV PGVSGKTFLN VVLDHAGHPE 421 AAYVPRRIEV VPYLAGKLGE GDLLITMGAG DVTAIGPQLV DALSQADGAA ARRQA //