LOCUS ACM57283.1 901 aa PRT BCT 01-MAR-2021 DEFINITION Halorubrum lacusprofundi ATCC 49239 DNA replication factor Dna2 protein. ACCESSION CP001365-1677 PROTEIN_ID ACM57283.1 SOURCE Halorubrum lacusprofundi ATCC 49239 ORGANISM Halorubrum lacusprofundi ATCC 49239 Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halorubrum. REFERENCE 1 (bases 1 to 2735295) AUTHORS Dassarma,S., Capes,M.D., Karan,R. and Dassarma,P. TITLE Amino acid substitutions in cold-adapted proteins from Halorubrum lacusprofundi, an extremely halophilic microbe from antarctica JOURNAL PLoS One 8 (3), e58587 (2013) PUBMED 23536799 REFERENCE 2 (bases 1 to 2735295) AUTHORS Karan,R., Capes,M.D., DasSarma,P. and DasSarma,S. TITLE Cloning, overexpression, purification, and characterization of a polyextremophilic beta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi JOURNAL BMC Biotechnol 13, 3 (2013) PUBMED 23320757 REMARK Publication Status: Online-Only REFERENCE 3 (bases 1 to 2735295) AUTHORS Anderson,I.J., DasSarma,P., Lucas,S., Copeland,A., Lapidus,A., Del Rio,T.G., Tice,H., Dalin,E., Bruce,D.C., Goodwin,L., Pitluck,S., Sims,D., Brettin,T.S., Detter,J.C., Han,C.S., Larimer,F., Hauser,L., Land,M., Ivanova,N., Richardson,P., Cavicchioli,R., DasSarma,S., Woese,C.R. and Kyrpides,N.C. TITLE Complete genome sequence of the Antarctic Halorubrum lacusprofundi type strain ACAM 34 JOURNAL Stand Genomic Sci 11 (1), 70 (2016) PUBMED 27617060 REMARK Publication Status: Online-Only REFERENCE 4 (bases 1 to 2735295) AUTHORS Laye,V.J., Karan,R., Kim,J.M., Pecher,W.T., DasSarma,P. and DasSarma,S. TITLE Key amino acid residues conferring enhanced enzyme activity at cold temperatures in an Antarctic polyextremophilic beta-galactosidase JOURNAL Proc Natl Acad Sci U S A 114 (47), 12530-12535 (2017) PUBMED 29109294 REFERENCE 5 (bases 1 to 2735295) AUTHORS Laye,V.J. and DasSarma,S. TITLE An Antarctic Extreme Halophile and Its Polyextremophilic Enzyme: Effects of Perchlorate Salts JOURNAL Astrobiology 18 (4), 412-418 (2018) PUBMED 29189043 REFERENCE 6 (bases 1 to 2735295) AUTHORS DasSarma,S., DasSarma,P., Laye,V.J. and Schwieterman,E.W. TITLE Extremophilic models for astrobiology: haloarchaeal survival strategies and pigments for remote sensing JOURNAL Extremophiles 24 (1), 31-41 (2020) PUBMED 31463573 REFERENCE 7 (bases 1 to 2735295) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (14-JAN-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4001421 Source DNA available from Professor Shiladitya DasSarma Archaea available from ATCC: ATCC 49239 Contacts: Ricardo Cavicchioli (r.cavicchioli@unsw.edu.au) Shiladitya DasSarma (sdassarma@som.umaryland.edu) David Bruce (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of at least two independent clones. Each base pair has a minimum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). FEATURES Qualifiers source /organism="Halorubrum lacusprofundi ATCC 49239" /mol_type="genomic DNA" /strain="ATCC 49239" /culture_collection="ATCC:49239" /type_material="type strain of Halorubrum lacusprofundi" /db_xref="taxon:416348" /chromosome="1" protein /locus_tag="Hlac_1698" /inference="protein motif:PFAM:PF08696" /note="PFAM: DNA replication factor Dna2; KEGG: nph:NP2944A helicase" /transl_table=11 /db_xref="InterPro:IPR014808" BEGIN 1 MTAVNVRGPI LSVGETRTVS TSYGDRELRE LRIRPDRGAG DAVDVTLWGK WTETAEHAEP 61 GMELLVTDAE EDEFGGETGY ATTGDSWVVL EPDFLVDVTG IRSWVQCPRM YYLNKLSGIP 121 LNYPVVKGTV VHEVFGDLLR GMELEDSVVD RVEEAGLELG LLGYEPEEVA DEVRRNAAAI 181 EGWLSQGTLT DEDTWRSEFT LISPTFGLKG RADALRRGTP VELKTGKNTK REPRFHDKIQ 241 AACYALMLEE RGVDPDIGTL LYTKNTALDR NEESGNLAPA KEFSVGQGLL QFVVRERNAL 301 AAMEWRALND RGERPTVPTG YEADAKCQYC FEQDTCMVVS GRLDQESKAG SVGTPVPNEE 361 RDYFDRFYVA LEEERRETHA EYRKLWEQTP EERAADDRAL IDLEPVSQTE IDDARWELRA 421 RKPGDAVSKL REGDVALASD GDPVTGHGEL GRITALSEDE VVVETDEPVE LRRLDVYPSE 481 ISVDRSLTAL HDAILKGSEA RKDVLFGRRE PEFRDAADRP ADAPEAYIDN NASQNEAVSL 541 AVDAEDGALI HGPPGTGKTY TIARTIRALV EEGNRVLLSA FTNRAVDNAL EALRDQGFDE 601 VLRVGSETGV RDDMQDVRLV QRGDPNAKAA ELRDAPVVAA TTAACGSRVL RECEFDVALV 661 DEASQLTEPG THAAINRADR FVLVGDHEQL PPVVRAENDL QTSLFQRLIE EYPDASVMLD 721 RQYRMSQRIQ AFASAEFYDG ALRPATPEVA GQTLRDLGVD PADLPDGLAG GVDFDDPDGT 781 RDGNRNVREA ERVAEIVDAY VAAGVDPDDI GVIAPFRAQV AEIGRRTAVT VDTVDRFQGS 841 SKEVIVVSLV ATGDLDGPIF EDHRRMNVAL TRAKKQLTLV GDDDALASEP FYARMLEWAQ 901 R //