LOCUS ACM56194.1 788 aa PRT BCT 01-MAR-2021 DEFINITION Halorubrum lacusprofundi ATCC 49239 phosphoenolpyruvate synthase protein. ACCESSION CP001365-588 PROTEIN_ID ACM56194.1 SOURCE Halorubrum lacusprofundi ATCC 49239 ORGANISM Halorubrum lacusprofundi ATCC 49239 Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halorubrum. REFERENCE 1 (bases 1 to 2735295) AUTHORS Dassarma,S., Capes,M.D., Karan,R. and Dassarma,P. TITLE Amino acid substitutions in cold-adapted proteins from Halorubrum lacusprofundi, an extremely halophilic microbe from antarctica JOURNAL PLoS One 8 (3), e58587 (2013) PUBMED 23536799 REFERENCE 2 (bases 1 to 2735295) AUTHORS Karan,R., Capes,M.D., DasSarma,P. and DasSarma,S. TITLE Cloning, overexpression, purification, and characterization of a polyextremophilic beta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi JOURNAL BMC Biotechnol 13, 3 (2013) PUBMED 23320757 REMARK Publication Status: Online-Only REFERENCE 3 (bases 1 to 2735295) AUTHORS Anderson,I.J., DasSarma,P., Lucas,S., Copeland,A., Lapidus,A., Del Rio,T.G., Tice,H., Dalin,E., Bruce,D.C., Goodwin,L., Pitluck,S., Sims,D., Brettin,T.S., Detter,J.C., Han,C.S., Larimer,F., Hauser,L., Land,M., Ivanova,N., Richardson,P., Cavicchioli,R., DasSarma,S., Woese,C.R. and Kyrpides,N.C. TITLE Complete genome sequence of the Antarctic Halorubrum lacusprofundi type strain ACAM 34 JOURNAL Stand Genomic Sci 11 (1), 70 (2016) PUBMED 27617060 REMARK Publication Status: Online-Only REFERENCE 4 (bases 1 to 2735295) AUTHORS Laye,V.J., Karan,R., Kim,J.M., Pecher,W.T., DasSarma,P. and DasSarma,S. TITLE Key amino acid residues conferring enhanced enzyme activity at cold temperatures in an Antarctic polyextremophilic beta-galactosidase JOURNAL Proc Natl Acad Sci U S A 114 (47), 12530-12535 (2017) PUBMED 29109294 REFERENCE 5 (bases 1 to 2735295) AUTHORS Laye,V.J. and DasSarma,S. TITLE An Antarctic Extreme Halophile and Its Polyextremophilic Enzyme: Effects of Perchlorate Salts JOURNAL Astrobiology 18 (4), 412-418 (2018) PUBMED 29189043 REFERENCE 6 (bases 1 to 2735295) AUTHORS DasSarma,S., DasSarma,P., Laye,V.J. and Schwieterman,E.W. TITLE Extremophilic models for astrobiology: haloarchaeal survival strategies and pigments for remote sensing JOURNAL Extremophiles 24 (1), 31-41 (2020) PUBMED 31463573 REFERENCE 7 (bases 1 to 2735295) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (14-JAN-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4001421 Source DNA available from Professor Shiladitya DasSarma Archaea available from ATCC: ATCC 49239 Contacts: Ricardo Cavicchioli (r.cavicchioli@unsw.edu.au) Shiladitya DasSarma (sdassarma@som.umaryland.edu) David Bruce (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of at least two independent clones. Each base pair has a minimum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). FEATURES Qualifiers source /organism="Halorubrum lacusprofundi ATCC 49239" /mol_type="genomic DNA" /strain="ATCC 49239" /culture_collection="ATCC:49239" /type_material="type strain of Halorubrum lacusprofundi" /db_xref="taxon:416348" /chromosome="1" protein /locus_tag="Hlac_0592" /inference="protein motif:TFAM:TIGR01418" /note="TIGRFAM: phosphoenolpyruvate synthase; PFAM: PEP-utilizing protein; pyruvate phosphate dikinase PEP/pyruvate-binding; PEP-utilising protein mobile region; KEGG: hwa:HQ1570A phosphoenolpyruvate synthase" /transl_table=11 /db_xref="InterPro:IPR000121" /db_xref="InterPro:IPR002192" /db_xref="InterPro:IPR006318" /db_xref="InterPro:IPR006319" /db_xref="InterPro:IPR008279" BEGIN 1 MAVLWLKDVD ADDVGTVGGK AASLGELIGA GLPVPPGFTV TAGTYRTFIE EAEIDEELFA 61 AVDVDPEDSV ALREAEETAA ELILETPLPD DVREEIVERY RTMGDGDDEA FVAVRSSATA 121 EDLPDSSFAG QQETFLNVRE QDLIRRVKEC WASLFTQRAI YYRQQRGFPH ADVDIAVVVQ 181 RMVDAEKSGV MFTSHPSTGD PQITIEAAWG LGEAVVSGTV SPDNYVYDRE RGAVDEVTVA 241 DKKVEMVKDA ETGETVTLEV DDERRNSRVL SDEEIAELVE LGKRVEDHYG TPQDVEWAIY 301 AGDIYMLQSR PITTIQEDAG SGRETDTGES AENIAGKDDV DDDTSDGVAA NGAGDRDDAS 361 DDADVLVNGL GASPGVVSGA VRIVHKLDHL DQVQEGDVMV TEMTMPDMVP AMKRAAGIIT 421 DEGGMTSHAA IISRELGVPA VVGTGNGTRV LNDGQNVTLD GDKGTVRAGA TADAESGEEF 481 EPVEAARPET PVKPMTATEV KVNVSIPEAA ERAAATGADG VGLLRIEHMV LSLGKTPEKY 541 IADHGARAYQ DELIEGVRRV ADEFYPRPVR VRTIDAPTDE FRELEGGEGE PAEPNPMLGW 601 RGIRRSLDKP EPFRQELAAF ARLYEMGYDN LEVMFPLVND ANDVEGIKRH MRESGIDPET 661 HRWGVMIETP ASALQIEDLA AAGIDFASFG TNDLTQYTLA VDRNNEHVAD RFDELHPAVL 721 QLIGDTIETC RELGVDTSIC GQAGSKPEMV DFLVEAGISS ISANIDAVRD VQHEVKRTEQ 781 RLLLDSVR //