LOCUS AAL19117.2 629 aa PRT BCT 24-JUL-2018 DEFINITION Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 pyruvate dehydrogenase protein. ACCESSION AE006468-153 PROTEIN_ID AAL19117.2 SOURCE Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ORGANISM Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Salmonella. REFERENCE 1 (bases 1 to 4857450) AUTHORS McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W., Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F., Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A., Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W., Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K. TITLE Complete genome sequence of Salmonella enterica serovar Typhimurium LT2 JOURNAL Nature 413 (6858), 852-856 (2001) PUBMED 11677609 REFERENCE 2 (bases 1 to 4857450) CONSRTM The Salmonella typhimurium Genome Sequencing Project TITLE Direct Submission JOURNAL Submitted (29-MAR-2001) Genome Sequencing Center, Department of Genetics, Washington University School of Medicine, 4444 Forest Park Boulevard, St. Louis, MO 63108, USA REFERENCE 3 (bases 1 to 4857450) AUTHORS McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R., SanMiguel,P. and Csonka,L. TITLE Direct Submission JOURNAL Submitted (13-JAN-2016) Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697, USA REMARK Sequence update by submitter COMMENT On Jan 13, 2016 this sequence version replaced AE006468.1. Supported by NIH grant 5U 01 AI43283 Coding sequences below are predicted from manually evaluated computer analysis, using similarity information and the programs; GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and GeneMark; http://opal.biology.gatech.edu/GeneMark/ EC numbers were kindly provided by Junko Yabuzaki and the Kyoto Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/, and Pedro Romero and Peter Karp at EcoCyc; http://ecocyc.PangeaSystems.com/ecocyc/ The analyses of ribosome binding sites and promoter binding sites were kindly provided by Heladia Salgado, Julio Collado-Vides and ReguonDB; http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistries or covered by high quality data (i.e., phred quality >= 30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by sequence from more than one m13 subclone. FEATURES Qualifiers source 2776826..2844430,2879238..4857450) /organism="Salmonella enterica subsp. enterica serovar Typhimurium str. LT2" /mol_type="genomic DNA" /strain="LT2" /serovar="Typhimurium" /sub_species="enterica" /culture_collection="ATCC:700720" /culture_collection="SGSC:1412" /type_material="type strain of Salmonella enterica" /db_xref="taxon:99287" /focus source /organism="Salmonella phage Fels-1" /mol_type="genomic DNA" /db_xref="taxon:128975" source /organism="Phage Gifsy-2" /mol_type="genomic DNA" /db_xref="taxon:129862" source /organism="Phage Gifsy-1" /mol_type="genomic DNA" /db_xref="taxon:129861" source /organism="Salmonella virus Fels2" /mol_type="genomic DNA" /db_xref="taxon:194701" protein /gene="aceF" /locus_tag="STM0153" /EC_number="2.3.1.12" /note="dihydrolipoyltransacetylase component; similar to E. coli pyruvate dehydrogenase (dihydrolipoyltransacetylase component) (AAC73226.1); Blastp hit to AAC73226.1 (630 aa), 93% identity in aa 1 - 630" /transl_table=11 BEGIN 1 MAIEIKVPDI GTDEVEITEI LVKVGDKVEA EQSLITVEGD KASMEVPSPQ AGVVKEIKVS 61 VGDKTETGAL IMIFDSAEGA ADAAPAKAEE KKEAAPAAAP AAAAAKDVHV PDIGSDEVEV 121 TEVMVKVGDT VEAEQSLITV EGDKASMEVP APFAGTVKEI KVNTGDKVST GSLIMVFEVA 181 GEAGAAAPAK AEAAPAAAAP AAATGVKDVN VPDIGGDEVE VTEVMVKVGD KVTAEQSLIT 241 VEGDKASMEV PAPFAGTVKE IKISTGDKVK TGSLIMVFEV EGAAPAAAPA KQEAAAPAPA 301 AKAEKPAAPA AKAEGKSEFA ENDAYVHATP LIRRLAREFG VNLAKVKGTG RKGRILREDV 361 QAYVKDAIKR AEAAPAAAGG GIPGMLPWPK VDFSKFGEVE EVELGRIQKI SGANLSRNWV 421 MIPHVTHFDK TDITDLEAFR KQQNAEAEKR KLDVKYTPVV FIMKAVAAAL EQMPRFNSSL 481 SEDGQRLTLK KYINIGVAVD TPNGLVVPVF KDVNKKSVTE LSRELTTISK KARDGKLTAG 541 EMQGGCFTIS SIGGLGTTHF APIVNAPEVA ILGVSKSAME PVWNGKEFVP RLMMPISLSF 601 DHRVIDGADG ARFITIINNM LSDIRRLVM //