LOCUS AAB59735.1 396 aa PRT SYN 30-SEP-2008 DEFINITION Cloning vector pBR322 tetracycline resistance protein protein. ACCESSION J01749-1 PROTEIN_ID AAB59735.1 SOURCE Cloning vector pBR322 ORGANISM Cloning vector pBR322 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3; 3259 to 4361) AUTHORS Sutcliffe,J.G. TITLE Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322 JOURNAL Proc. Natl. Acad. Sci. U.S.A. 75 (8), 3737-3741 (1978) PUBMED 358200 REFERENCE 2 (bases 1 to 4361) AUTHORS Sutcliffe,J.G. TITLE Complete nucleotide sequence of the Escherichia coli plasmid pBR322 JOURNAL Cold Spring Harb. Symp. Quant. Biol. 43 (Pt 1), 77-90 (1979) PUBMED 383387 REFERENCE 3 (bases 1500 to 2300) AUTHORS Reed,R.R., Young,R.A., Steitz,J.A., Grindley,N.D. and Guyer,M.S. TITLE Transposition of the Escherichia coli insertion element gamma generates a five-base-pair repeat JOURNAL Proc. Natl. Acad. Sci. U.S.A. 76 (10), 4882-4886 (1979) PUBMED 388421 REFERENCE 4 (bases 2207 to 2265) AUTHORS Covarrubias,L., Cervantes,L., Covarrubias,A., Soberon,X., Vichido,I., Blanco,A., Kupersztoch-Portnoy,Y.M. and Bolivar,F. TITLE Construction and characterization of new cloning vehicles. V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328 JOURNAL Gene 13 (1), 25-35 (1981) PUBMED 6263753 REFERENCE 5 (bases 2000 to 2500) AUTHORS Marians,K.J., Soeller,W. and Zipursky,S.L. TITLE Maximal limits of the Escherichia coli replication factor Y effector site sequences in pBR322 DNA JOURNAL J. Biol. Chem. 257 (10), 5656-5662 (1982) PUBMED 6279609 REFERENCE 6 (bases 1 to 80; 4151 to 4229; 4349 to 4361) AUTHORS Brosius,J., Cate,R.L. and Perlmutter,A.P. TITLE Precise location of two promoters for the beta-lactamase gene of pBR322. S1 mapping of ribonucleic acid isolated from Escherichia coli or synthesized in vitro JOURNAL J. Biol. Chem. 257 (15), 9205-9210 (1982) PUBMED 6178738 REFERENCE 7 (bases 4241 to 4343) AUTHORS Van Dyke,M.W., Hertzberg,R.P. and Dervan,P.B. TITLE Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II) JOURNAL Proc. Natl. Acad. Sci. U.S.A. 79 (18), 5470-5474 (1982) PUBMED 6291045 REFERENCE 8 (bases 584 to 709) AUTHORS Peden,K.W. and Nathans,D. TITLE Local mutagenesis within deletion loops of DNA heteroduplexes JOURNAL Proc. Natl. Acad. Sci. U.S.A. 79 (23), 7214-7217 (1982) PUBMED 6760191 REFERENCE 9 (bases 373 to 649) AUTHORS Peden,K.W. TITLE Revised sequence of the tetracycline-resistance gene of pBR322 JOURNAL Gene 22 (2-3), 277-280 (1983) PUBMED 6307828 REFERENCE 10 (bases 132 to 181) AUTHORS Watabe,H., Iino,T., Kaneko,T., Shibata,T. and Ando,T. TITLE A new class of site-specific endodeoxyribonucleases. Endo.Sce I isolated from a eukaryote, Saccharomyces cerevisiae JOURNAL J. Biol. Chem. 258 (8), 4663-4665 (1983) PUBMED 6300094 REFERENCE 11 (bases 368 to 581) AUTHORS Livneh,Z. TITLE Directed mutagenesis method for analysis of mutagen specificity: application to ultraviolet-induced mutagenesis JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80 (1), 237-241 (1983) PUBMED 6337373 REFERENCE 12 (bases 2627 to 2682; 2781 to 2828) AUTHORS Mascharak,P.K., Sugiura,Y., Kuwahara,J., Suzuki,T. and Lippard,S.J. TITLE Alteration and activation of sequence-specific cleavage of DNA by bleomycin in the presence of the antitumor drug cis-diamminedichloroplatinum(II) JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80 (22), 6795-6798 (1983) PUBMED 6196777 REFERENCE 13 (bases 4276 to 4336) AUTHORS Schultz,P.G. and Dervan,P.B. TITLE Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA X Fe(II) JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80 (22), 6834-6837 (1983) PUBMED 6417654 REFERENCE 14 (bases 518 to 528) AUTHORS Sutcliffe,J.G. JOURNAL Unpublished REFERENCE 15 (bases 2395 to 2495) AUTHORS Fuller,R.S., Funnell,B.E. and Kornberg,A. TITLE The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites JOURNAL Cell 38 (3), 889-900 (1984) PUBMED 6091903 REFERENCE 16 (bases 2729 to 2731) AUTHORS Lathe,R., Kieny,M.P., Skory,S. and Lecocq,J.P. TITLE Linker tailing: unphosphorylated linker oligonucleotides for joining DNA termini JOURNAL DNA 3 (2), 173-182 (1984) PUBMED 6327214 REFERENCE 17 (bases 2729 to 2730) AUTHORS Heusterspreute,M. and Davison,J. TITLE Restriction site bank vectors. II. DNA sequence analysis of plasmid pJRD158 JOURNAL DNA 3 (3), 259-268 (1984) PUBMED 6086259 REFERENCE 18 (bases 2113 to 2186; 2348 to 2415) AUTHORS Abarzua,P., Soeller,W. and Marians,K.J. TITLE Mutational analysis of primosome assembly sites. I. Distinct classes of mutants in the pBR322 Escherichia coli factor Y DNA effector sequences JOURNAL J. Biol. Chem. 259 (22), 14286-14292 (1984) PUBMED 6209275 REFERENCE 19 (bases 2348 to 2415) AUTHORS Soeller,W., Abarzua,P. and Marians,K.J. TITLE Mutational analysis of primosome assembly sites. II. Role of secondary structure in the formation of active sites JOURNAL J. Biol. Chem. 259 (22), 14293-14300 (1984) PUBMED 6150042 REFERENCE 20 (bases 1 to 4361) AUTHORS Van Dyke,M.M. and Dervan,P.B. TITLE Echinomycin binding sites on DNA JOURNAL Science 225 (4667), 1122-1127 (1984) PUBMED 6089341 REFERENCE 21 (sites) AUTHORS Pouwels,P.H., Enger-Valk,B.E. and Brammar,W.J. JOURNAL (in) CLONING VECTORS. Elsevier Scientific Publishing, Amsterdam (1985) REMARK Vector I-A-iv-1 REFERENCE 22 (bases 1 to 4361) AUTHORS Watson,N. TITLE A new revision of the sequence of plasmid pBR322 JOURNAL Gene 70 (2), 399-403 (1988) PUBMED 3063608 REFERENCE 23 (sites) AUTHORS Gilbert,W. TITLE Obtained from VecBase 3.0 JOURNAL Unpublished COMMENT On or before Apr 4, 2002 this sequence version replaced L08654.1, gi:58257. The circular sequence is numbered such that 0 is the middle of the unique EcoRI site and the count increases first through the tet genes, the pMB1 material, and finally through the Tn3 region. Plasmid pBR322 contains ampicillin and tetracycline resistance genes. The ampicillin resistance gene (amp-r) is a penicillin beta-lactamase. Promoters P1 and P3 are for the beta-lactamase gene. P3 is the natural promoter, and P1 is artificially created by the ligation of two different DNA fragments to create pBR322. P2 is in the same region as P1, but it is on the opposite strand and initiates transcription in the direction of the tetracycline resistance gene. Mutational studies in the primosome assembly sites indicate four types of mutations: Class I having no effect on the activities elicited by the DNA site and the bases involved are probably spacers; Class II requiring higher Mg-2+ concentrations than the wild-type to be fully activated as factor Y ATPase effectors; Class III co-inactivating both the ATPase effector and DNA replication template activity of the site, indicating that they probably represent essential contact points between factor Y and the DNA; Class IV having a replication template activity intermediate that of class III and class II mutant DNAs. Specific sites within or near the origins of replication are recognized by dnaA protein. Without dnaA binding to the origin of replication chromosomal replication is not possible (15). pBR322 DNA contains two separate regions on opposite strands and close to the origin of replication which, when in single-stranded form, can act as effectors for the ATPase activity of E.coli replication factor Y (5). Small fragments of DNA containing these sites when cloned in an f1 phage vector act as origins of DNA replication allowing the formation of complementary double-stranded DNA in rifampicin-resistant, dna(B,G,C)-dependent fashion in vitro (5). The biological activity of echinomycin is thought to be related to the formation of complexes by intercalating with cellular DNA (20). Complete source information: Plasmid pBR322 from E.coli (2),(1),(3),(6),(11),(8),(5),(7),(12), (13),(10),(9),(14),(18),(19),(15),(20),(16); pBR322 DNA in pXf3 (4). The following data and their annotation were supplied by Will Gilbert under the auspices of the Curator Program. CROSSREFERENCE #parent GenBank(50):pSC101C, GenBank(50):Trn3 #offspring VecBase(3):pBR325, VecBase(3):pBR327, VecBase(3):pBR328, VecBase(3):pAT153, VecBase(3):pUC7, VecBase(3):pJRD158, VecBase(3):PiVX, VecBase(3):PiAN7, VecBase(3):pSP64, VecBase(3):pSP65, VecBase(3):pGEM1, VecBase(3):pGEM2, VecBase(3):pGEM3, VecBase(3):pGEM4, VecBase(3):pKK223, VecBase(3):pLBU3, VecBase(3):pTrS3, VecBase(3):pRSVNeo, VecBase(3):pSV2Cat, VecBase(3):M13mp9, VecBase(3):pHC79, VecBase(3):pV34, VecBase(3):pKTH601, VecBase(3):pKTH604, VecBase(3):pKTH605, VecBase(3):pKTH606, VecBase(3):YEp24, VecBase(3):YIp5, VecBase(3):YRp17, VecBase(3):pSP18, VecBase(3):pSP19, VecBase(3):pSP6T3, VecBase(3):pSP6T719, VecBase(3):pT712, VecBase(3):pT713, VecBase(3):pT7T318, VecBase(3):pT7T319, VecBase(3):pT7T3A18, VecBase(3):pT7T3A19, VecBase(3):pEX1, VecBase(3):pEX2, VecBase(3):pEX3, VecBase(3):pCKSP6, VecBase(3):pACYC177, VecBase(3):pKO1, VecBase(3):pKO2, VecBase(3):pKM1, VecBase(3):pKM2, VecBase(3):pMBL1, VecBase(3):pMBL604, VecBase(3):pMC1511, VecBase(3):pMC1871, VecBase(3):pAA37X, VecBase(3):pUR278, VecBase(3):pUR288, VecBase(3):pUR289, VecBase(3):pUR290, VecBase(3):pUR291, VecBase(3):pUR292, VecBase(3):pUR222. FEATURES Qualifiers source /organism="Cloning vector pBR322" /mol_type="other DNA" /db_xref="taxon:47470" /tissue_lib="ATCC 31344, ATCC 37017" /focus source /organism="Plasmid pSC101" /mol_type="other DNA" /db_xref="taxon:2625" protein /gene="tet" /transl_table=11 BEGIN 1 MKSNNALIVI LGTVTLDAVG IGLVMPVLPG LLRDIVHSDS IASHYGVLLA LYALMQFLCA 61 PVLGALSDRF GRRPVLLASL LGATIDYAIM ATTPVLWILY AGRIVAGITG ATGAVAGAYI 121 ADITDGEDRA RHFGLMSACF GVGMVAGPVA GGLLGAISLH APFLAAAVLN GLNLLLGCFL 181 MQESHKGERR PMPLRAFNPV SSFRWARGMT IVAALMTVFF IMQLVGQVPA ALWVIFGEDR 241 FRWSATMIGL SLAVFGILHA LAQAFVTGPA TKRFGEKQAI IAGMAADALG YVLLAFATRG 301 WMAFPIMILL ASGGIGMPAL QAMLSRQVDD DHQGQLQGSL AALTSLTSIT GPLIVTAIYA 361 ASASTWNGLA WIVGAALYLV CLPALRRGAW SRATST //