LOCUS       AAB59347.1               565 aa    PRT              PLN 06-APR-1996
DEFINITION  Saccharomyces cerevisiae damage-repair protein protein.
ACCESSION   M37696-4
PROTEIN_ID  AAB59347.1
SOURCE      Saccharomyces cerevisiae (brewer's yeast)
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 249 to 352)
  AUTHORS   Montgomery,D.L., Hall,B.D., Gillam,S. and Smith,M.
  TITLE     Identification and isolation of the yeast cytochrome c gene
  JOURNAL   Cell 14 (3), 673-680 (1978)
   PUBMED   210956
REFERENCE   2  (bases 1 to 857)
  AUTHORS   Smith,M., Leung,D.W., Gillam,S., Astell,C.R., Montgomery,D.L. and
            Hall,B.D.
  TITLE     Sequence of the gene for iso-1-cytochrome c in Saccharomyces
            cerevisiae
  JOURNAL   Cell 16 (4), 753-761 (1979)
   PUBMED   222467
REFERENCE   3  (bases 190 to 299)
  AUTHORS   Stiles,J.I., Szostak,J.W., Young,A.T., Wu,R., Consaul,S. and
            Sherman,F.
  TITLE     DNA sequence of a mutation in the leader region of the yeast
            iso-1-cytochrome c mRNA
  JOURNAL   Cell 25 (1), 277-284 (1981)
   PUBMED   6268305
REFERENCE   4  (bases 188 to 280; 576 to 753)
  AUTHORS   Boss,J.M., Gillam,S., Zitomer,R.S. and Smith,M.
  TITLE     Sequence of the yeast iso-1-cytochrome c mRNA
  JOURNAL   J. Biol. Chem. 256 (24), 12958-12961 (1981)
   PUBMED   6273415
REFERENCE   5  (bases 1 to 260)
  AUTHORS   Faye,G., Leung,D.W., Tatchell,K., Hall,B.D. and Smith,M.
  TITLE     Deletion mapping of sequences essential for in vivo transcription
            of the iso-1-cytochrome c gene
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 78 (4), 2258-2262 (1981)
   PUBMED   6264471
REFERENCE   6  (bases 576 to 765)
  AUTHORS   Zaret,K.S. and Sherman,F.
  TITLE     DNA sequence required for efficient transcription termination in
            yeast
  JOURNAL   Cell 28 (3), 563-573 (1982)
   PUBMED   6280875
REFERENCE   7  (sites)
  AUTHORS   Ernst,J.F., Hampsey,D.M. and Sherman,F.
  TITLE     DNA sequences of frameshift and other mutations induced by ICR-170
            in yeast
  JOURNAL   Genetics 111 (2), 233-241 (1985)
   PUBMED   2414151
REFERENCE   8  (bases 249 to 578)
  AUTHORS   Hampsey,D.M., Das,G. and Sherman,F.
  TITLE     Amino acid replacements in yeast iso-1-cytochrome c. Comparison
            with the phylogenetic series and the tertiary structure of related
            cytochromes c
  JOURNAL   J. Biol. Chem. 261 (7), 3259-3271 (1986)
   PUBMED   3005287
REFERENCE   9  (bases 771 to 8241)
  AUTHORS   Melnick,L. and Sherman,F.
  TITLE     Nucleotide sequence of the COR region: a cluster of six genes in
            the yeast Saccharomyces cerevisiae
  JOURNAL   Gene 87 (2), 157-166 (1990)
   PUBMED   2158927
COMMENT     Original source text: Saccharomyces cerevisiae (clone:  pAB40) DNA.
            Dr. Graeme A. Reid (ebmo21@castle.edinburgh.ac.uk) writes us about
            the possibility of a sequence error, a deletion of a c at position
            4617-4618 (cc->ccc), which would extend the coding region to
            position 5225 instead of 4683.   He made the following
            observations: This sequence is homologous to Shewanella
            putrefaciens flavocytochrome c (L04283) and other fumarate
            reductases. The present GenBank entry indicates that OSM1 encodes a
            protein of 301 residues.  Extremely close sequence similarity
            between OSM1 protein and flavocytochrome c beyond the end point was
            found in another reading frame.  It is possible that a sequencing
            error accounts for this observation, but it has not been checked
            experimentally as yet.       The putative signal sequence of the
            OSM1 protein does not fit the consensus at all well and there is no
            evidence to suggest that this is a membrane protein or enters the
            secretory pathway.  The proposed correction would restore the
            sequebnce QxHPTG that is well conserved throughout the Frd family.
            with the histidine in the sequence having been identified at the
            active site in E.coli frdA (Schryder et al., 1991). Wild-type yeast
            cells have two cytochrome c proteins, iso-1 and iso-2, encoded by
            the two distinct genetic loci cyc1 and cyc7, respectively.  The
            iso-1 cytochrome c protein is the predominant cellular cytochrome
            c, normally representing about 95% of the material of that kind.
            The cyc1-512 mutant has a 38 bp deletion in the 3' nontranslated
            region which prevents proper transcription termination.  Authors
            suggest that polyadenylation may be coupled to transcription
            termination in yeast.  They also report a consensus sequence from
            many yeast genes.
FEATURES             Qualifiers
     source          /organism="Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:4932"
                     /clone="pAB40"
     protein         /gene="RAD7"
                     /note="UV-induced"
                     /db_xref="SGD:L0001561"
BEGIN
        1 MYRSRNRPKR GGENEVKGPN SALTQFLREE GISAENIKQK WYQRQSKKQE DATDEKKGKA
       61 EDDSFTAEIS RVVEDEEIDE IGTGSGTETE RAQVSYDARM KLVPADSDEE EYETSHISDT
      121 PVSLSSANDR ESLTKKRQNT AKIIQNRRRK RKRAADLLDR RVNKVSSLQS LCITKISENI
      181 SKWQKEADES SKLVFNKLRD VLGGVSTANL NNLAKALSKN RALNDHTLQL FLKTDLKRLT
      241 FSDCSKISFD GYKTLAIFSP HLTELSLQMC GQLNHESFVY IAEKLPNLKS LNLDGPFLIN
      301 EDTWEKFFVI MKGRLEEFHI SNTHRFTDKS LSNLLINCGS TLVSLGLSRL DSISNYALLP
      361 QYLVNDEFHS LCIEYPFNEE DVNDEIIINL LGQIGRTLRK LVLNGCIDLT DSMIINGLTA
      421 FIPEKCPLEV LSLEESDQIT TDSLSYFFSK VELNNLIECS FRRCLQLGDM AIIELLLNGA
      481 RDSLRSLNLN SLKELTKEAF VALRPPNLTY LDLGFVRCVD DSVIQMLGEQ NPNLTVIDVF
      541 GDNLVTEKAT MRPGLTLIGR QSDSI
//