LOCUS       AAA86441.1               149 aa    PRT              BCT 01-FEB-1996
DEFINITION  Bacillus amyloliquefaciens barnase (RNase) precursor protein.
ACCESSION   M14442-1
PROTEIN_ID  AAA86441.1
SOURCE      Bacillus amyloliquefaciens
  ORGANISM  Bacillus amyloliquefaciens
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Paddon,C.J. and Hartley,R.W.
  TITLE     Cloning, sequencing and transcription of an inactivated copy of
            Bacillus amyloliquefaciens extracellular ribonuclease (barnase)
  JOURNAL   Gene 40 (2-3), 231-239 (1985)
   PUBMED   3007290
REFERENCE   2  (bases 181 to 420)
  AUTHORS   Paddon,C.J., Vasantha,N. and Hartley,R.W.
  TITLE     Translation and processing of Bacillus amyloliquefaciens
            extracellular RNase
  JOURNAL   J. Bacteriol. 171 (2), 1185-1187 (1989)
   PUBMED   2914867
REFERENCE   3  (bases 1 to 779)
  AUTHORS   Hartley,R.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-1988) Robert W. Hartley, LCDB/NIDDK, National
            Institutes of Health, Bethesda, MD 20892, USA
REFERENCE   4  (bases 1 to 832)
  AUTHORS   Hartley,R.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-1996) Robert W. Hartley, LCDB/NIDDK, National
            Institutes of Health, Bethesda, MD 20892, USA
COMMENT     On Feb 1, 1996 this sequence version replaced gi:142614.
            [2]  translation start sites.
            Pro I barnase is cleaved to form pro II barnase (positions 391-735)
            after which it is cleaved again to form the mature peptide.
FEATURES             Qualifiers
     source          /organism="Bacillus amyloliquefaciens"
                     /mol_type="genomic DNA"
                     /strain="H2R1"
                     /db_xref="taxon:1390"
                     /map="195 bp upstream of RsaI site"
                     /clone="pMT6"
     protein         /transl_table=11
BEGIN
        1 MKKRLSWISV CLLVLVSAAG MLFSTAAKTE TSSHKAHTEA QVINTFDGVA DYLQTYHKLP
       61 DNYITKSEAQ ALGWVASKGN LADVAPGKSI GGDIFSNREG KLPGKSGRTW READINYTSG
      121 FRNSDRILYS SDWLIYKTTD HYQTFTKIR
//